- Chromatography: Blotting paper is often used in chromatography techniques, such as paper chromatography or thin-layer chromatography, to separate and analyze different components of a mixture based on their differential solubility and migration rates.
- Drying of Samples: Blotting paper is used to dry wet or damp samples, especially in biological and chemical research. It can be placed on top of the sample to absorb excess moisture without disturbing the sample itself.
- Sample Storage: In certain cases, blotting paper can be used to store samples like dried plant specimens or fragile materials that need to be preserved for later analysis.
- Absorbent Pads: Blotting paper is used as an absorbent pad in various laboratory applications, including in microbiology to blot excess liquid from bacterial cultures during colony transfer or in enzyme assays to remove excess reagents.
- Cleaning Glassware: Blotting paper is used to clean and dry glassware in the lab, ensuring that residual moisture is removed, which can be critical for accurate measurements and experiments.
- Absorption of Chemical Spills: Blotting paper can be used to quickly absorb small chemical spills on surfaces. It helps to contain and clean up spills efficiently while minimizing the spread of hazardous substances.
- Support for Drying Gels: In some cases, blotting paper is used as a support for drying gels like agarose gels after electrophoresis.
- Moisture Barrier: Blotting paper can be used as a barrier to prevent the transfer of moisture between layers of materials, which can be important in preserving sensitive samples.
- Protein Blot Staining and Detection: In protein blotting procedures like Western blots, blotting paper is used to hold the membrane and filter paper layers together during incubation with antibodies and detection reagents.