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Disodium Hydrogen Phosphate 500gm

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Disodium phosphate, also known as sodium hydrogen phosphate or disodium hydrogen phosphate, is an inorganic compound with the chemical formula Na2HPO4. It is a white, crystalline powder or granular solid that is highly soluble in water.

In terms of its chemical composition, disodium phosphate consists of two sodium (Na+) ions and one phosphate (PO43-) ion. It is derived from phosphoric acid and is commonly used as a food additive, pH buffer, and a source of phosphorus in various industries.

As a food additive, disodium phosphate is primarily used as a texturizer, emulsifier, and stabilizer in processed foods and beverages. It helps improve the texture, increase shelf life, and prevent the separation of ingredients. Additionally, it can act as a pH regulator in certain food products.

In summary, disodium phosphate is an inorganic compound that serves various purposes in food and industrial applications, primarily as a food additive and pH buffer.

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Disodium Hydrogen Phosphate.

 

In laboratory settings, disodium hydrogen phosphate (Na2HPO4) finds several important uses:

  1. Buffer Solutions: Disodium hydrogen phosphate is commonly used as a component in buffer solutions, especially those used in biological and biochemical experiments. Buffer solutions help maintain a constant pH level, which is crucial for many reactions and experiments.
  2. DNA and Protein Electrophoresis: In molecular biology and biochemistry laboratories, disodium hydrogen phosphate is used in gel electrophoresis procedures for separating DNA or proteins based on their size and charge. It helps in the preparation of running buffers for these procedures.
  3. Cell Culture Media: In cell culture applications, disodium hydrogen phosphate can be included in the formulation of cell culture media to maintain a stable pH environment for cell growth and proliferation.
  4. Analytical Chemistry: Disodium hydrogen phosphate is used as a reagent in various analytical chemistry techniques, such as spectrophotometry and chromatography, for sample preparation, calibration, and standardization.
  5. Titration: In titration experiments, disodium hydrogen phosphate can be used as a standard solution or as a titrant to determine the concentration of acids or bases in a given solution.
  6. Phosphate Analysis: It is also used in the analysis of phosphate levels in environmental samples, such as water and soil, as well as in biological samples, to assess phosphate content or to study phosphate metabolism.
  7. Protein Purification: Disodium hydrogen phosphate can be utilized in protein purification procedures, such as ion exchange chromatography, as a component of the buffer system for protein elution and separation.
SKU: ACS79665CHEM0 Category:
Weight 500 kg

When working with disodium hydrogen phosphate (Na2HPO4) in laboratory settings, it’s important to observe safety precautions to minimize potential risks. Here are some safety guidelines:

  1. Personal Protective Equipment (PPE): Wear appropriate PPE, including lab coat, safety goggles, and gloves, when handling disodium hydrogen phosphate to prevent skin contact and eye irritation.
  2. Handling: Handle disodium hydrogen phosphate with care to avoid inhalation of dust or contact with skin and eyes. Use tools such as spatulas or scoops rather than bare hands when transferring the compound.
  3. Ventilation: Work in a well-ventilated area or under a fume hood to minimize exposure to airborne dust or vapors.
  4. Storage: Store disodium hydrogen phosphate in tightly sealed containers in a dry and well-ventilated area away from incompatible substances. Follow any specific storage recommendations provided by the manufacturer.
  5. Spill Cleanup: In case of spills, carefully clean up the material using appropriate absorbent materials and dispose of it according to local regulations. Avoid creating dust and wear appropriate PPE during cleanup.
  6. First Aid: In case of accidental exposure (ingestion, inhalation, or skin/eye contact), immediately flush affected areas with plenty of water and seek medical attention if necessary. Have a readily accessible eyewash station and emergency shower in the laboratory.
  7. Disposal: Dispose of disodium hydrogen phosphate and any contaminated materials according to local regulations and institutional guidelines for chemical waste disposal.
  8. Chemical Compatibility: Be aware of chemical compatibility when handling disodium hydrogen phosphate. Avoid contact with incompatible substances, such as strong acids or oxidizing agents, to prevent hazardous reactions.
  9. Labeling: Ensure that containers of disodium hydrogen phosphate are properly labeled with appropriate hazard warnings and safety information.
  10. Training: Provide training to laboratory personnel on the safe handling, storage, and disposal of disodium hydrogen phosphate, as well as emergency procedures in case of accidents or spills.

 

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