Folin Ciocalteus Phenol Reagent 2.5l

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Folin Ciocalteus phenol reagent is a chemical solution commonly used in analytical chemistry to determine the concentration of phenolic compounds, particularly in plant extracts and food samples. The reagent is named after its developers, Otto Folin and Vintilă Ciocâlteu.

The Folin-Ciocalteu reagent consists of a mixture of phosphomolybdic and phosphotungstic acids in a highly alkaline solution (usually sodium carbonate or sodium hydroxide). When this reagent comes into contact with phenolic compounds, such as polyphenols, flavonoids, and other aromatic compounds, a blue-colored complex forms due to a reduction of the phosphomolybdic-phosphotungstic acid mixture by the phenols. The intensity of the blue color is proportional to the concentration of phenolic compounds in the sample.

This reagent is widely used for the quantification of total phenolic content in various biological and food samples, making it an essential tool in antioxidant and phytochemical research. The Folin-Ciocalteu assay is a reliable and well-established method for assessing the antioxidant capacity of samples and has been utilized in numerous scientific studies and analytical applications.


Folin Ciocalteus phenol reagent has several major and common uses in analytical chemistry, particularly in the assessment of phenolic compounds in various samples. Some of the main applications include:

  1. Total phenolic content (TPC) determination: The Folin-Ciocalteu reagent is widely used to quantify the total phenolic content in plant extracts, foods, beverages, and other biological samples. This is an essential tool in antioxidant and phytochemical research, as phenolic compounds often contribute significantly to the antioxidant capacity of these samples.
  2. Antioxidant activity assessment: By measuring the total phenolic content, the Folin-Ciocalteu assay allows researchers to evaluate the antioxidant activity of different samples. Antioxidants play a crucial role in protecting cells from oxidative damage and are important in the context of health and disease.
  3. Polyphenol analysis in foods and beverages: The reagent is employed to determine the polyphenol content in various food and beverage products. This information is relevant for assessing the nutritional value and potential health benefits of these products.
  4. Quality control in the food industry: The Folin-Ciocalteu assay is used in quality control processes to ensure consistent phenolic content in food and beverage products. It helps manufacturers maintain product quality and meet regulatory standards.
  5. Phytochemical screening: In drug discovery and natural product research, the reagent is used to screen plant extracts and identify potential bioactive compounds rich in phenolic content.
  6. Environmental analysis: The Folin-Ciocalteu reagent can be applied to assess the phenolic content in environmental samples, such as water and soil, to monitor pollution levels and environmental health.
  7. Analyzing wine and beer: The reagent is commonly used in the wine and beer industries to determine the total phenolic content, which is relevant for assessing their quality, flavor, and aging potential.
SKU: ACS19236CHEM0 Category:

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