Folin Ciocalteus Phenol Reagent 2.5l

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Folin Ciocalteus phenol reagent is a chemical solution commonly used in analytical chemistry to determine the concentration of phenolic compounds, particularly in plant extracts and food samples. The reagent is named after its developers, Otto Folin and Vintilă Ciocâlteu.

The Folin-Ciocalteu reagent consists of a mixture of phosphomolybdic and phosphotungstic acids in a highly alkaline solution (usually sodium carbonate or sodium hydroxide). When this reagent comes into contact with phenolic compounds, such as polyphenols, flavonoids, and other aromatic compounds, a blue-colored complex forms due to a reduction of the phosphomolybdic-phosphotungstic acid mixture by the phenols. The intensity of the blue color is proportional to the concentration of phenolic compounds in the sample.

This reagent is widely used for the quantification of total phenolic content in various biological and food samples, making it an essential tool in antioxidant and phytochemical research. The Folin-Ciocalteu assay is a reliable and well-established method for assessing the antioxidant capacity of samples and has been utilized in numerous scientific studies and analytical applications.


Folin Ciocalteus Phenol Reagent


Folin-Ciocalteu’s phenol reagent is widely used in various laboratory settings due to its effectiveness in quantifying phenolic compounds. Here are some specific laboratory uses:

1. Food and Beverage Analysis

  • Total Phenolic Content (TPC): Determining the TPC in fruits, vegetables, juices, wines, teas, and other food products to assess their antioxidant capacity and nutritional value.
  • Quality Control: Ensuring the quality and consistency of food products by monitoring phenolic content, which can affect flavor, color, and health benefits.

2. Plant and Agricultural Research

  • Phytochemical Screening: Analyzing plant extracts for phenolic compounds, which are important for understanding plant biochemistry, ecology, and potential medicinal properties.
  • Breeding Programs: Selecting plant varieties with higher phenolic content for improved health benefits and disease resistance.

3. Pharmaceutical and Cosmetic Industries

  • Herbal Extracts: Measuring phenolic content in herbal and botanical extracts used in dietary supplements and traditional medicines.
  • Cosmetic Formulations: Analyzing phenolic compounds in cosmetic products for their antioxidant properties, which can protect skin from damage and aging.

4. Environmental Science

  • Soil and Water Analysis: Determining phenolic compounds in soil and water samples to study environmental pollution and the effects of phenolic pollutants on ecosystems.
  • Waste Treatment: Monitoring phenolic content in industrial effluents and wastewater to ensure compliance with environmental regulations.

5. Biomedical Research

  • Antioxidant Studies: Investigating the antioxidant properties of various compounds and extracts, which can contribute to the development of new therapeutic agents.
  • Nutritional Studies: Analyzing the phenolic content in dietary supplements and functional foods to understand their health benefits.

6. Biochemical and Molecular Biology

  • Enzyme Activity Assays: Using the reagent to measure the activity of enzymes that oxidize phenolic compounds, such as polyphenol oxidases and peroxidases.
  • Metabolomics: Studying the phenolic profile of biological samples to understand metabolic pathways and their regulation.
SKU: ACS19236CHEM0 Category:

When working with Folin-Ciocalteu’s phenol reagent in the laboratory, it is essential to follow safety precautions to ensure a safe working environment. Here are some key safety guidelines:

Personal Protective Equipment (PPE)

  • Gloves: Wear appropriate chemical-resistant gloves (e.g., nitrile gloves) to prevent skin contact.
  • Lab Coat: Wear a lab coat to protect your clothing and skin from spills and splashes.
  • Safety Goggles: Use safety goggles or face shields to protect your eyes from potential splashes.
  • Closed-Toe Shoes: Wear closed-toe shoes to protect your feet from spills.

Handling and Storage

  • Handling:
    • Work in a well-ventilated area or fume hood to avoid inhaling fumes.
    • Avoid direct contact with the reagent; handle with care to prevent spills and splashes.
    • Use appropriate containers for mixing and reacting to prevent breakage and spills.
  • Storage:
    • Store the Folin-Ciocalteu reagent in a cool, dry, and well-ventilated place.
    • Keep the reagent in tightly closed containers to prevent contamination and evaporation.
    • Store away from incompatible materials, such as strong acids, bases, and oxidizing agents.

Spill and Accident Procedures

  • Spills:
    • In the event of a small spill, use absorbent materials (e.g., paper towels or absorbent pads) to clean up.
    • For larger spills, evacuate the area and follow your laboratory’s spill response procedures.
    • Dispose of contaminated materials according to local regulations and guidelines.
  • Skin Contact:
    • Immediately wash the affected area with plenty of water and soap.
    • Remove contaminated clothing and seek medical attention if irritation persists.
  • Eye Contact:
    • Rinse eyes thoroughly with water for at least 15 minutes, keeping eyelids open.
    • Seek immediate medical attention.
  • Inhalation:
    • Move to fresh air and seek medical attention if respiratory symptoms occur.
  • Ingestion:
    • Rinse mouth with water (do not induce vomiting) and seek immediate medical attention.

Waste Disposal

  • Chemical Waste:
    • Collect waste containing the Folin-Ciocalteu reagent in a designated chemical waste container.
    • Label the container clearly with its contents and hazard information.
    • Dispose of waste according to your institution’s hazardous waste disposal guidelines and local regulations.
  • Contaminated Materials:
    • Dispose of gloves, absorbent materials, and other contaminated items in designated hazardous waste containers.

General Safety Practices

  • Training: Ensure all personnel working with the reagent are properly trained in its use and the associated safety precautions.
  • Labeling: Clearly label all containers with the contents and appropriate hazard warnings.
  • Documentation: Maintain Safety Data Sheets (SDS) for the Folin-Ciocalteu reagent and ensure they are readily accessible.
  • Emergency Equipment: Ensure that emergency eyewash stations and safety showers are available and functional in the laboratory.

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