Folin Ciocalteus Phenol Reagent 2.5l

Folin Ciocalteus Phenol Reagent 2.5l

The Folin-Ciocalteu phenol reagent is a chemical solution commonly used in analytical chemistry to determine the concentration of phenolic compounds, particularly in plant extracts and food samples. The reagent is named after its developers, Otto Folin and Vintilă Ciocâlteu.

The Folin-Ciocalteu reagent consists of a mixture of phosphomolybdic and phosphotungstic acids in a highly alkaline solution (usually sodium carbonate or sodium hydroxide). When this reagent comes into contact with phenolic compounds, such as polyphenols, flavonoids, and other aromatic compounds, a blue-colored complex forms due to a reduction of the phosphomolybdic-phosphotungstic acid mixture by the phenols. The intensity of the blue color is proportional to the concentration of phenolic compounds in the sample.

This reagent is widely used for the quantification of total phenolic content in various biological and food samples, making it an essential tool in antioxidant and phytochemical research. The Folin-Ciocalteu assay is a reliable and well-established method for assessing the antioxidant capacity of samples and has been utilized in numerous scientific studies and analytical applications.



The Folin-Ciocalteu phenol reagent has several major and common uses in analytical chemistry, particularly in the assessment of phenolic compounds in various samples. Some of the main applications include:

  1. Total phenolic content (TPC) determination: The Folin-Ciocalteu reagent is widely used to quantify the total phenolic content in plant extracts, foods, beverages, and other biological samples. This is an essential tool in antioxidant and phytochemical research, as phenolic compounds often contribute significantly to the antioxidant capacity of these samples.
  2. Antioxidant activity assessment: By measuring the total phenolic content, the Folin-Ciocalteu assay allows researchers to evaluate the antioxidant activity of different samples. Antioxidants play a crucial role in protecting cells from oxidative damage and are important in the context of health and disease.
  3. Polyphenol analysis in foods and beverages: The reagent is employed to determine the polyphenol content in various food and beverage products. This information is relevant for assessing the nutritional value and potential health benefits of these products.
  4. Quality control in the food industry: The Folin-Ciocalteu assay is used in quality control processes to ensure consistent phenolic content in food and beverage products. It helps manufacturers maintain product quality and meet regulatory standards.
  5. Phytochemical screening: In drug discovery and natural product research, the reagent is used to screen plant extracts and identify potential bioactive compounds rich in phenolic content.
  6. Environmental analysis: The Folin-Ciocalteu reagent can be applied to assess the phenolic content in environmental samples, such as water and soil, to monitor pollution levels and environmental health.
  7. Analyzing wine and beer: The reagent is commonly used in the wine and beer industries to determine the total phenolic content, which is relevant for assessing their quality, flavor, and aging potential.

Overall, the Folin-Ciocalteu phenol reagent is a versatile tool in the analysis of phenolic compounds, playing a significant role in various scientific disciplines, including biochemistry, food science, pharmacology, and environmental science. Its ability to quantify and assess phenolic content and antioxidant activity makes it a widely used and reliable method for researchers and analysts.


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Safety Guidelines

When using the Folin-Ciocalteu phenol reagent, it is essential to observe certain precautions to ensure accurate and safe results. Here are some precautions to consider:
  1. Safety gear: Wear appropriate personal protective equipment, including lab coats, gloves, and safety goggles, to protect against potential chemical splashes or spills.
  2. Handle with care: The Folin-Ciocalteu reagent is corrosive and can cause skin and eye irritation. Avoid direct contact with the skin, eyes, and clothing. In case of contact, immediately rinse the affected area with plenty of water and seek medical attention if necessary.
  3. Work in a well-ventilated area: Perform the assay in a fume hood or well-ventilated area to avoid inhalation of fumes and to minimize exposure to volatile components.
  4. Avoid contamination: Keep all glassware, pipettes, and other equipment used in the assay clean and free from any residues that could contaminate the reagent or the sample.
  5. Accurate measurements: Use accurate and calibrated measuring instruments (such as pipettes and burettes) to ensure precise volumes of reagents and samples are added.
  6. Proper storage: Store the Folin-Ciocalteu reagent in a cool, dry place, away from direct sunlight and incompatible substances. Follow the manufacturer’s recommendations for storage conditions.
  7. Standardization: Calibrate and standardize the reagent before each use to ensure consistent and reliable results. Prepare fresh reagent if necessary, as old or degraded reagent may lead to inaccurate measurements.
  8. Work with appropriate concentrations: Use the Folin-Ciocalteu reagent at the recommended concentration for your specific assay. Dilute or concentrate the reagent if needed, following established protocols.
  9. Proper waste disposal: Dispose of any unused or waste reagent according to local regulations and waste disposal guidelines.
  10. Understand the reaction mechanism: Familiarize yourself with the chemical reaction that occurs between the Folin-Ciocalteu reagent and phenolic compounds to interpret the results correctly.
  11. Perform blank and control tests: Always include appropriate blank and control samples to account for background absorbance and verify the accuracy of the assay.
  12. Record keeping: Maintain clear and detailed records of the procedure, reagents used, and results obtained to ensure reproducibility and traceability of your experiments.
By observing these precautions, you can minimize potential risks, ensure accurate results, and work safely with the Folin-Ciocalteu phenol reagent in your laboratory.

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