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Rhodamine B
Rhodamine B is a bright pink to reddish-purple synthetic dye belonging to the xanthene family. It is widely used as a fluorescent tracer dye and coloring agent due to its strong fluorescence and high photostability. Rhodamine B finds applications in analytical chemistry, biological staining, and industrial processes requiring vivid coloration or fluorescence detection.
Rhodamine B
Primary Uses
- Analytical Chemistry and Fluorescence Tracing
- Used as a fluorescent tracer dye in water flow studies, leak detection, and dye tracing in environmental research.
- Applied in fluorescence microscopy and flow cytometry for staining and detecting biological samples.
- Textile Industry
- Utilized as a dye for coloring synthetic fibers, nylon, and polyester fabrics.
- Provides bright and durable coloration in fabric dyeing processes.
- Biological and Medical Research
- Employed as a staining agent for cells and tissues in histology and cytology.
- Used in fluorescence-based assays and imaging techniques to visualize biological structures.
Secondary Uses
- Ink and Paint Manufacturing
- Incorporated in inks and paints to produce vivid fluorescent colors.
- Laser Dyes and Optics
- Used in laser technology as a gain medium in dye lasers.
- Plastics and Polymers
- Added to plastics and resins to impart fluorescent properties for identification or decoration.
1. Basic Identification Attributes
- Chemical Name (IUPAC): [9-(2-carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]diethylammonium chloride
- Common/Trade Name: Rhodamine B
- CAS Number: 81-88-9
- HS Code: 3204.17
- Synonyms: Basic Violet 10, C.I. 45170
2. Physical & Chemical Properties
- Physical State: Powder or solid crystals
- Color & Odor: Dark pink to reddish-purple; odorless
- Solubility: Soluble in water and ethanol
- Melting Point: Approx. 228°C (decomposes)
- Fluorescence: Strong pink-red fluorescence under UV light
3. Safety & Hazard Attributes
- GHS Classification: Harmful if swallowed, suspected carcinogen
- Toxicity: Moderate toxicity; handle with care
- Exposure Limits: Use with appropriate safety measures
4. Storage & Handling Attributes
- Storage Conditions: Store in tightly closed containers, away from light and moisture
- Container Type: Sealed plastic or glass containers
- Shelf Life: Stable if stored properly in a cool, dry place
- Handling Precautions: Avoid inhalation and skin contact; use protective equipment
5. Regulatory & Compliance Attributes
- Subject to chemical safety regulations
- Use restricted or regulated in some countries due to toxicity concerns
6. Environmental & Health Impact
- Biodegradability: Not readily biodegradable
- Ecotoxicity: Toxic to aquatic organisms
- Bioaccumulation: Potential for bioaccumulation
Safety Handling Precautions
- PPE Required: Gloves, goggles, protective clothing, and dust mask or respirator
- Handling Guidelines: Use in well-ventilated areas; avoid dust generation and direct contact
- Storage Measures: Keep containers tightly closed and stored in a dry environment away from light
First Aid Measures
- Inhalation: Move to fresh air; seek medical attention if respiratory irritation occurs
- Skin Contact: Wash with soap and water; seek medical advice if irritation persists
- Eye Contact: Rinse with water for at least 15 minutes; seek medical care if irritation continues
- Ingestion: Rinse mouth; do not induce vomiting; seek immediate medical help
Firefighting Measures
- Fire Hazards: Combustible solid
- Extinguishing Media: Use water spray, foam, dry chemical, or CO₂
- Special Precautions: Wear full protective gear and self-contained breathing apparatus if involved in fire
- Hazardous Combustion Products: Carbon oxides, nitrogen oxides
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