Sudan 3 Solution 2.5 liters

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Chemical Name: 1-(2,5-Dimethyl-4-phenylazo)-2-naphthol

Molecular Formula: C22H16N2O

Appearance: Red to brownish-red powder

Solubility: Soluble in ethanol, benzene, and oils; slightly soluble in water

Preparation of Sudan III Solution:

  • Stock Solution: Typically, a stock solution is prepared by dissolving 0.5g of Sudan III in 100ml of 70% ethanol.
  • Working Solution: For staining, the stock solution can be diluted with ethanol or isopropanol to the desired concentration. A common working concentration is 0.5% Sudan III in ethanol or isopropanol.


Sudan 3 Solution

Sudan III is used in a variety of laboratory settings for specific staining applications, particularly related to the detection and visualization of lipids. Here are the primary uses:

  1. Histology:
    • Lipid Staining in Tissue Sections: Sudan III stains lipids in tissue sections, especially in frozen sections, which helps in identifying and visualizing lipid droplets within cells and tissues. This is useful for diagnosing conditions like fatty liver disease and lipid storage disorders.
  2. Cytology:
    • Cell Smears: It stains lipids in cell smears, aiding in examining cells for lipid content. This is important in diagnosing metabolic conditions or infections involving lipid accumulation.
  3. Forensic Pathology:
    • Detection of Fat Emboli: Sudan III is used to detect fat emboli in tissues, which can be crucial in cases of trauma, decompression sickness, or other conditions where fat emboli may be present.
  4. Plant Biology:
    • Analysis of Seed Lipids: In plant biology, Sudan III stains lipids in seeds and other plant tissues, helping in the study of lipid storage and metabolism in plants.
  5. Microbiology:
    • Staining of Bacterial Lipids: Sudan III can stain certain types of bacteria that contain lipid-rich cell walls, aiding in their identification and characterization.
  6. Clinical Diagnostics:
    • Identifying Lipid-Rich Deposits: It is used in clinical settings to identify lipid-rich deposits in various tissues, indicative of diseases such as atherosclerosis or lipid storage diseases.
  7. Veterinary Medicine:
    • Lipid Analysis in Animal Tissues: Sudan III can be used to analyze lipid content in animal tissues, assisting in diagnosing lipid-related conditions in veterinary practice.


SKU: ACS76142CHEM0 Category:

When working with Sudan III, it is important to follow safety precautions to minimize risks associated with handling this chemical. Here are key safety measures:

  1. Personal Protective Equipment (PPE):
    • Gloves: Wear protective gloves to avoid skin contact.
    • Goggles: Use safety goggles to protect your eyes from splashes.
    • Lab Coat: Wear a lab coat or apron to protect your clothing and skin.
  2. Ventilation:
    • Fume Hood: Perform all work involving Sudan III in a well-ventilated area, preferably under a fume hood, to avoid inhaling fumes.
    • Adequate Airflow: Ensure the workspace has adequate airflow to disperse any vapors.
  3. Handling:
    • Avoid Inhalation: Do not breathe in dust or fumes. Use respiratory protection if ventilation is inadequate.
    • Minimize Exposure: Avoid contact with skin and eyes. Handle the chemical with care to prevent spills and splashes.
  4. Storage:
    • Proper Containers: Store Sudan III in tightly closed containers to prevent leakage.
    • Dark, Cool Place: Keep the chemical in a dark, cool place away from direct sunlight and heat sources to prevent degradation.
    • Labeling: Clearly label the containers with the chemical name and hazard information.
  5. First Aid Measures:
    • Skin Contact: In case of skin contact, immediately wash with plenty of soap and water. Remove contaminated clothing.
    • Eye Contact: If the chemical gets into the eyes, rinse thoroughly with water for at least 15 minutes and seek medical attention.
    • Inhalation: If inhaled, move the person to fresh air. If breathing is difficult, seek medical attention immediately.
    • Ingestion: If swallowed, do not induce vomiting. Rinse mouth with water and seek medical attention immediately.
  6. Spill and Disposal Procedures:
    • Spills: In case of a spill, use absorbent materials (e.g., paper towels, vermiculite) to clean up and dispose of the waste properly. Clean the area with soap and water.
    • Disposal: Dispose of Sudan III and contaminated materials according to local, regional, or national regulations. Do not pour it down the drain or throw it in regular trash.
  7. Fire Safety:
    • Flammability: Sudan III is flammable. Keep it away from open flames, sparks, and other sources of ignition.
    • Fire Extinguisher: Have an appropriate fire extinguisher (e.g., CO2, dry chemical) readily available in case of a fire.
  8. Training and Information:
    • Safety Data Sheet (SDS): Familiarize yourself with the SDS for Sudan III. It provides detailed information on handling, hazards, and emergency measures.
    • Training: Ensure that all personnel handling Sudan III are trained in proper safety procedures and emergency response.

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