Benedict Solution Extra Pure

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Benedict Solution Extra Pure is a high-purity, blue-colored reagent solution widely used in laboratory settings for the qualitative detection of reducing sugars, such as glucose and fructose. It contains copper(II) sulfate, sodium citrate, and sodium carbonate, which react with reducing sugars under heat to form a colored precipitate ranging from green to brick red, depending on sugar concentration. This makes it essential in biochemical and educational laboratories for carbohydrate analysis and metabolic studies. The extra pure grade ensures consistent composition and reliable colorimetric response, supporting accurate and reproducible results in teaching and research environments. It should be stored in a cool, dark place in a tightly sealed container to maintain stability and prevent degradation.

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Categories: Analytical Reagents, Biochemical Reagents, microbiology-and-cell-culture-reagents Tags: BenedictSolution, BiochemicalReagent, CarbohydrateTest, ColorChangeTest, GlucoseDetection, HighPurityReagent, LabReagent, ReducingSugars

Description

USES

Primary Uses

Detection of Reducing Sugars
- Benedict’s solution is widely used to test for the presence of reducing sugars (e.g., glucose, fructose, maltose) in qualitative carbohydrate analysis.
- Upon heating with a reducing sugar, it forms a brick-red precipitate of cuprous oxide (Cu₂O), indicating a positive result.
Educational Demonstrations in Carbohydrate Chemistry
- Commonly used in school and university teaching labs to demonstrate color change reactions, oxidation-reduction, and sugar classification.
Semi-Quantitative Estimation of Sugar Levels
- In some cases, the intensity of the color change (blue → green → yellow → orange → red) is used to estimate approximate sugar concentration in a sample.

Secondary Uses

Urinalysis Simulations in Clinical Chemistry Training
- Used in lab simulations for clinical biochemistry, especially to mimic testing for glucosuria (presence of glucose in urine).
Teaching Redox Chemistry
- Demonstrates redox principles, where Cu²⁺ (blue) is reduced to Cu⁺ (red precipitate), and the sugar is oxidized — ideal for oxidation-reduction education.
Comparison with Other Sugar Reagents
- In comparative studies with Fehling’s solution, Tollen’s reagent, or Barfoed’s reagent, it helps differentiate mono- and disaccharides, or reducing vs. non-reducing sugars.
Baseline Control in Analytical Method Development
- Serves as a reference reagent in developing or validating newer methods of sugar detection in complex matrices.

Product Key Features

KEY ATTRIBUTES

1. Basic Identification Attributes

Chemical Name: Benedict's Solution
CAS Number: Mixture (no single CAS; components have individual CAS numbers)
HS Code: 38229090 (Prepared diagnostic or laboratory reagents)
Molecular Formula: Mixture — primarily contains:
- Copper(II) sulfate pentahydrate (CuSO₄·5H₂O)
- Sodium carbonate (Na₂CO₃)
- Sodium citrate (C₆H₅Na₃O₇)
Synonyms:
- Benedict Reagent
- Benedict Qualitative Solution
- Benedict's Test Solution

2. Physical & Chemical Properties

Physical State: Aqueous solution
Color & Odor: Blue liquid; odorless or mildly alkaline odor
Boiling Point: Approx. 100 °C (water-based)
Melting Point: Not applicable
Density: ~1.05–1.10 g/cm³
Solubility: Completely miscible in water
pH Level: Mildly alkaline (around pH 9–10)
Vapor Pressure: Similar to water
Flash Point: Not flammable
Autoignition Temperature: Not applicable
Viscosity: Low (water-like)

3. Safety & Hazard Attributes

GHS Classification:
- Not classified as hazardous
- Copper salts may be harmful if ingested in large quantities
NFPA Ratings:
- Health: 1
- Flammability: 0
- Reactivity: 0
Exposure Limits:
- OSHA PEL (Copper dust/mist): 1 mg/m³ (TWA)
Reactivity:
- Stable under normal lab conditions
- Reacts with strong acids (releases CO₂)

4. Storage & Handling Attributes

Storage Conditions:
- Store tightly sealed at room temperature (15–25 °C)
- Avoid light and contamination
Incompatible Materials:
- Strong acids
- Reducing agents (may cause unwanted reactions)
Container Type:
- Amber glass or HDPE/plastic lab bottles
Shelf Life:
- Typically 1–2 years in sealed containers
Special Handling:
- Wear gloves and lab coat
- Wash skin thoroughly after handling
- Use in a well-ventilated area

5. Regulatory & Compliance Attributes

Regulatory Status:
- Not regulated under DOT or IATA
- Components like CuSO₄ are listed in REACH and TSCA
Hazard Symbols (GHS):
- May include exclamation mark if high copper concentration
Transportation Restrictions:
- Not classified as hazardous for transport
Waste Disposal:
- Dispose as chemical waste
- Avoid disposal in drains due to copper content
- Follow local environmental regulations

6. Environmental & Health Impact

Ecotoxicity:
- Copper component is toxic to aquatic life
Persistence:
- Copper persists in the environment; must be disposed of properly
Carcinogenicity/Mutagenicity:
- Not classified as carcinogenic
Biodegradability:
- Inorganic; not biodegradable

Safety Handling Precautions

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):
- Wear lab coat, chemical-resistant gloves, and safety goggles.
- Use a face shield if splashing is likely.
Handling:
- Handle in a well-ventilated area or fume hood.
- Avoid contact with skin and eyes; avoid inhaling vapors or mist.
- Stir gently during heating to prevent spattering.
Storage:
- Store in a cool, dry, and dark place.
- Keep the container tightly closed when not in use.
- Avoid storing near strong acids or oxidizers.
Hygiene Measures:
- Wash hands thoroughly after handling.
- Avoid eating or drinking in the lab.

FIRST AID MEASURES

Inhalation:
- Remove victim to fresh air.
- Get medical advice if symptoms persist.
Skin Contact:
- Wash thoroughly with water and soap.
- Remove contaminated clothing.
Eye Contact:
- Rinse immediately with plenty of water for at least 15 minutes.
- Seek medical help if irritation persists.
Ingestion:
- Rinse mouth with water.
- Do not induce vomiting.
- Seek medical attention immediately.

FIRE FIGHTING MEASURES

Extinguishing Media:
- Use CO₂, foam, dry chemical, or water spray.
Fire Hazards:
- Benedict’s Solution is not highly flammable, but components like sodium citrate and copper sulfate may decompose upon heating, releasing:
  - Copper fumes
  - Irritating or toxic gases (e.g., SOx, COx)
Protective Equipment:
- Use self-contained breathing apparatus (SCBA) and protective clothing.
Firefighting Instructions:
- Cool containers with water spray.
- Prevent runoff from entering drains.