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Bromothymol Blue Extra Pure

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Bromothymol Blue Extra Pure is a high-purity pH indicator dye widely used in laboratory experiments, especially in acid-base titrations and biological research. It exhibits a distinct color change from yellow in acidic solutions (pH < 6.0) to blue in basic conditions (pH > 7.6), making it valuable for monitoring pH changes in aqueous systems. This dye is also employed in respiratory experiments to detect CO₂ levels through pH shifts. The extra pure grade ensures excellent color stability, high sensitivity, and minimal impurities, making it suitable for precise analytical and educational applications. It should be stored in a tightly closed container, protected from light and moisture to maintain its integrity.

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Description

Primary Uses
pH Indicator in Acid–Base Titrations

Changes from yellow (pH < 6.0) to blue (pH > 7.6)—ideal for titrations near neutral pH (e.g., weak acid vs. strong base).

Buffer System Studies

Used in buffer capacity experiments and to visually monitor pH stability in buffer solutions during kinetic or enzymatic studies.

Respiration and Photosynthesis Experiments

Applied in biology labs to detect CO₂ levels:

Yellow in acidic solutions (CO₂ dissolved)

Blue in basic or neutral solutions

Helps monitor cellular respiration or photosynthetic CO₂ uptake.

Colorimetric Assays and Visual pH Checks

Incorporated into colorimetric kits for rapid visual estimation of pH, especially in soil, water, and biochemical media testing.

Secondary Uses
Demonstration of pH Concept in Teaching Labs

Great for classroom demonstrations showing pH shifts and acid-base neutralization visually.

Biological Culture Monitoring

Sometimes added to cell culture media or fermentation broths to visually signal pH changes due to microbial metabolism.

Spectrophotometric Calibration

Used in UV-Vis experiments for studying absorbance spectra of indicators, or calibrating spectrophotometers.

PRODUCT KEY FEATURES

Basic Identification Attributes
Chemical Name: Bromothymol Blue

CAS Number: 76-59-5

HS Code: 2934.99.90

Molecular Formula: C₂₇H₂₈Br₂O₅S

Molecular Weight: 624.38 g/mol

Synonyms: BTB, Bromothymol sulfone phthalein

Grade: Extra Pure (for analytical/laboratory reagent use)

2. Physical & Chemical Properties
Appearance: Dark green to bluish powder or crystals

Odor: Odorless

Solubility: Slightly soluble in water; soluble in ethanol and dilute alkali solutions

pH Range (Color Change):

Yellow below pH 6.0

Green at pH ~7.0

Blue above pH 7.6

Melting Point: Decomposes above 200 °C (no clear melting point)

Stability: Stable under normal lab conditions when kept dry and sealed

3. Safety & Hazard Attributes
GHS Classification:

Not classified as hazardous under GHS in low quantities

GHS Pictograms: None required for typical lab-scale use

NFPA Ratings:

Health: 1

Flammability: 0

Reactivity: 0

Health Hazards:

May cause mild irritation to eyes, skin, or respiratory tract

Protective Measures:

Use lab gloves, goggles, and standard ventilation

4. Storage & Handling Attributes
Storage Conditions:

Store in a tightly sealed container, in a cool, dry, dark place

Protect from moisture and strong oxidizers

Incompatible Materials:

Strong acids, oxidizers

Packaging: Amber glass or HDPE bottles with desiccants

Handling Precautions:

Avoid dust formation; handle with care to maintain reagent purity

5. Regulatory & Compliance Attributes
Regulatory Status:

Listed under TSCA and other chemical inventories

Transport Classification:

Not regulated for transport under standard laboratory quantities

Waste Disposal:

Small quantities may be disposed of in regular lab chemical waste

Follow local institutional chemical disposal guidelines

6. Environmental & Health Impact
Ecotoxicity:

Low ecological risk at laboratory scale

Biodegradability:

Slowly degrades in the environment

Bioaccumulation:

Not expected to bioaccumulate

Health Effects:

Minimal unless ingested or in case of prolonged exposure

SAFETY HANDLING PRECAUTIONS

Personal Protective Equipment (PPE):

Wear safety goggles, lab gloves (nitrile recommended), and a lab coat.

Handle powder in a fume hood to avoid inhalation.

Handling:

Avoid generation of dust.

Prevent contact with skin, eyes, and clothing.

Wash hands thoroughly after use.

Storage:

Store in a cool, dry, and well-ventilated area.

Keep the container tightly closed and protected from light.

Keep away from incompatible materials such as strong oxidizing agents.

Incompatibilities:

Avoid mixing with strong acids or oxidizers.

FIRST AID MEASURES
Inhalation:

Remove to fresh air.

Seek medical attention if symptoms such as coughing or shortness of breath occur.

Skin Contact:

Wash immediately with plenty of water and soap.

Remove contaminated clothing.

Seek medical advice if irritation persists.

Eye Contact:

Rinse thoroughly with plenty of water for 15 minutes, lifting eyelids.

Seek medical attention if irritation or vision issues persist.

Ingestion:

Rinse mouth with water.

Do not induce vomiting.

Get medical attention, especially if large amounts were swallowed.

FIRE FIGHTING MEASURES
Flammability:

Not classified as flammable, but may burn if exposed to fire.

Extinguishing Media:

Use water spray, foam, carbon dioxide, or dry chemical.

Hazardous Combustion Products:

May release carbon oxides (CO, CO₂) and sulfur oxides under fire conditions.

Firefighting Equipment:

Use self-contained breathing apparatus (SCBA) and protective gear.

Cool containers with water if exposed to fire.

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