Carmine Aceto Extra Pure

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Carmine Aceto Extra Pure is a high-purity, bright red dye derived from carminic acid, used extensively in laboratory applications involving histological staining, pH indicators, and biochemical assays. Known for its strong color stability in acidic environments, it is especially valuable in microscopy for highlighting cell structures and tissue components. Its precise formulation makes it suitable for use in analytical techniques requiring consistent coloration and minimal contamination. The extra pure grade ensures superior quality and reproducibility in experimental conditions. It should be stored in a cool, dry place, protected from direct light and moisture to preserve its dyeing efficacy and stability.

Description

Primary Uses
Biological Staining Agent

Widely used in microscopy as a nuclear stain to highlight cell nuclei and chromosomes, particularly in histology and cytology.

Aceto-Carmine Staining in Plant Cytogenetics

Essential for preparing root tip squash slides to observe mitosis and meiosis phases in plant cells (e.g., onion root tips).

Chromosome Visualization in Mitosis Studies

Used in educational and research labs for clear observation of metaphase chromosomes, especially in plant cytology and genetics labs.

Teaching & Demonstration Reagent

Standard dye in biology classroom experiments to demonstrate nuclear division.

Secondary Uses
Reference Dye in Comparative Staining Techniques

Occasionally used to compare with other nuclear stains (e.g., acetoorcein) during method optimization in staining protocols.

Slide Preparation Quality Control

Used to test fixation and staining consistency in microscopy slide prep techniques.

Training in Laboratory Techniques

Integral in lab training programs for preparing, fixing, and staining tissue samples.

PRODUCT KEY FEATURES

Basic Identification Attributes
Chemical Name: Carmine Aceto

Grade: Extra Pure (Laboratory Grade)

Type: Natural dye derivative

Synonyms: Acetous Carmine, Aceto-Carmine Solution

2. Physical & Chemical Properties
Appearance: Deep red to reddish-violet liquid or powder (depending on form)

Odor: Mild vinegar-like (if in solution)

Solubility: Soluble in water and ethanol

pH (for solution): Typically acidic (due to presence of acetic acid)

Composition: Typically a solution of carmine dye in acetic acid and/or ethanol

3. Safety & Hazard Attributes
Hazard Classification: Generally low hazard; may contain acetic acid

Hazard Statements (if applicable):

May cause mild skin or eye irritation

Inhalation of dust (if powdered) should be avoided

Precautionary Statements:

Use gloves and eye protection

Work in a well-ventilated area

Avoid ingestion and inhalation

4. Storage & Handling Attributes
Storage Conditions:

Store tightly sealed in a cool, dry place away from light

If in solution, store in amber bottles to protect from light degradation

Handling Precautions:

Use standard laboratory PPE (gloves, goggles, lab coat)

Clean spills immediately; may stain surfaces

5. Regulatory & Compliance Attributes
Transport: Not classified as hazardous for transport

Waste Disposal: Dispose according to local chemical waste regulations

REACH / OSHA / GHS: Not classified as hazardous under most chemical regulations

6. Environmental & Health Impact
Toxicity: Very low toxicity; used in some biological stains and food coloring historically

Health Effects:

Minimal; may cause irritation with prolonged contact

Environmental Hazards: Not considered environmentally hazardous in small lab quantities

Fumes/Dusts: Avoid inhaling fine powder (if applicable); solution form poses minimal inhalation risk

SAFETY HANDLING PRECAUTIONS

Personal Protective Equipment (PPE):

Wear lab coat, nitrile gloves, and safety goggles.

Use in a well-ventilated area or fume hood to avoid inhalation of dust.

Avoid direct contact with skin or eyes.

Handling:

Handle as a fine powder—avoid generating dust.

Prevent contamination with incompatible substances such as strong acids or oxidizers.

Use clean tools and containers to prevent reactions or degradation.

Storage:

Store in a cool, dry, dark place in tightly sealed containers.

Protect from moisture, heat, and light.

Keep away from oxidizing agents and strong acids.

FIRST AID MEASURES
Inhalation:

Move to fresh air.

If irritation or symptoms persist, seek medical attention.

Skin Contact:

Wash with plenty of soap and water.

Remove contaminated clothing.

Seek medical advice if irritation occurs.

Eye Contact:

Rinse cautiously with water for several minutes.

If irritation develops or persists, seek medical attention.

Ingestion:

Rinse mouth.

Do not induce vomiting.

Seek medical advice if large quantities are ingested or if symptoms appear.

FIRE FIGHTING MEASURES
Flammability:

May be combustible as a fine powder.

Avoid dust buildup near ignition sources.

Extinguishing Media:

Use dry chemicals, COâ‚‚, or foam.

Water spray may be used to cool containers.

Hazardous Combustion Products:

Carbon oxides and nitrogen oxides may form under fire conditions.

Firefighter Protection:

Wear SCBA (self-contained breathing apparatus) and protective clothing.