Diastase Enzyme Powder Extra Pure

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SHORT DESCRIPTION

Diastase Enzyme Powder Extra Pure is a high-activity amylolytic enzyme preparation used in laboratory settings to catalyze the breakdown of starch into simpler sugars like maltose and glucose. It plays a crucial role in biochemical and enzymatic assays, fermentation studies, and educational demonstrations involving carbohydrate metabolism. This extra pure grade ensures high enzyme purity, offering reliable and reproducible results in analytical work and research. Ideal for controlled hydrolysis experiments and studies on enzyme kinetics, it should be stored in a cool, dry place to preserve its enzymatic activity and stability.

Description

USES

Primary Uses

  1. Starch Hydrolysis Experiments 
    • Diastase catalyzes the breakdown of starch into maltose and glucose, ideal for teaching enzyme kinetics and carbohydrate metabolism. 
  2. Biochemical Studies 
    • Used in assays to study amylolytic activity, enzyme specificity, and optimal conditions (pH, temperature) for enzymatic action. 
  3. Pharmaceutical Testing 
    • Included in digestive enzyme formulations and evaluated for enzyme activity levels under various storage and formulation conditions. 

Secondary Uses

  • Food Chemistry Labs – Demonstrates enzymatic conversion of starches in beer brewing, baking, and fermentation science. 
  • Histochemistry – Occasionally used in tissue digestion protocols before staining procedures. 
  • Comparative Enzyme Studies – Paired with amylase, papain, or cellulase for multi-enzyme reaction demonstrations.
KEY PRODUCT FEATURES

KEY ATTRIBUTES

1. Basic Identification Attributes

  • Chemical Name: Diastase 
  • Other Name(s): Amylase (Mixture of α- and β-amylase enzymes) 
  • CAS Number: 9000-92-4 
  • Grade: Extra Pure (Laboratory Grade) 
  • Appearance: Off-white to light beige powder 
  • Source: Typically derived from malted grain or microbial fermentation 

2. Physical & Chemical Properties

  • Solubility: Soluble in water; insoluble in alcohol 
  • Enzymatic Activity: Hydrolyzes starch into maltose and dextrins 
  • pH Range of Activity: Optimal at pH 4.6–6.0 
  • Temperature Stability: Active at 30 °C to 70 °C (denatures above ~80 °C) 
  • Odor: Slight fermentation-like or neutral 

3. Safety & Hazard Attributes

  • GHS Classification: May cause respiratory sensitization (Category 1) 
  • Hazard Statements: 
    • H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled 
  • Precautionary Measures: 
    • Avoid inhalation of enzyme dust 
    • Use appropriate PPE: gloves, goggles, dust mask or respirator 
    • Avoid contact with mucous membranes 
  • First Aid: 
    • Inhalation: Move to fresh air; seek medical help if symptoms persist 
    • Skin Contact: Wash with soap and water 
    • Eye Contact: Rinse thoroughly with water 
    • Ingestion: Rinse mouth; seek medical attention if large quantities are consumed 

4. Storage & Handling Attributes

  • Storage Conditions: 
    • Store in a cool, dry, tightly sealed container 
    • Protect from heat, light, and moisture 
  • Shelf Life: Typically 1–2 years when stored properly 
  • Handling Advice: 
    • Avoid creating airborne dust 
    • Use in a well-ventilated area or fume hood for bulk handling 

5. Regulatory & Compliance Attributes

  • EC Number: 232-565-6 
  • REACH Status: Exempt (naturally derived enzyme) 
  • Transport Classification: Not regulated as hazardous 

6. Laboratory Applications

  • Primary Uses: 
    • Starch hydrolysis experiments in biology and biochemistry 
    • Enzymatic digestion in food and fermentation analysis 
    • Quality control testing in brewing and distilling 
    • Demonstrations in enzymology and kinetics labs 
  • Other Uses: 
    • Brewing industry (mashing process) 
    • Diagnostic reagent in biolabs 
    • Educational enzyme activity experiments
SAFETY HANDLING PRECAUTIONS

SAFETY MEASURES

LABORATORY SAFETY & HANDLING

  • PPE Required: 
    • Dust mask or respirator (enzyme powders can be allergenic) 
    • Gloves 
    • Lab coat 
    • Safety goggles 
  • Storage Guidelines: 
    • Store in a cool, dry place (preferably 2–8°C in sealed container) 
    • Protect from moisture, heat, and direct sunlight 
    • Avoid repeated freeze-thaw cycles if stored in solution 
  • Handling Tips: 
    • Avoid inhalation of fine powder – work in a well-ventilated area or fume hood 
    • Clean spills promptly to prevent airborne enzyme particles 
    • Prevent contamination by using clean spatulas and containers 

FIRST AID MEASURES

  • Inhalation: Move to fresh air; seek medical help if respiratory symptoms develop 
  • Skin Contact: Wash thoroughly with soap and water 
  • Eye Contact: Flush eyes with water for at least 15 minutes; seek medical advice 
  • Ingestion (Lab Use): Rinse mouth, do not induce vomiting; seek medical attention 

FIRE SAFETY MEASURES

  • Fire Hazard: Not flammable under normal conditions 
  • Explosion Risk: Fine dust may form combustible mixtures with air 
  • Extinguishing Agents: Water spray, CO₂, dry chemical 
  • Combustion Products: May produce carbon oxides (CO, CO₂)