Dipotassium Hydrogen Phosphate Extra Pure
SHORT DESCRIPTION
Dipotassium Hydrogen Phosphate Extra Pure is a high-purity inorganic salt commonly used in laboratories as a buffering agent, particularly in the preparation of phosphate buffer solutions for biochemical and analytical procedures. It plays a vital role in maintaining stable pH conditions in enzymatic and molecular biology experiments. Additionally, it serves as a source of potassium and phosphorus in various chemical reactions and culture media. This extra pure grade ensures excellent consistency and low levels of contaminants, making it ideal for high-precision lab work. It should be stored in a cool, dry place, tightly sealed to avoid moisture absorption.
USES
Primary Uses
- Buffer Preparation
- Commonly used to make phosphate buffer solutions in biochemistry, molecular biology, and analytical chemistry.
- pH Control
- Acts as a mildly basic buffering agent to stabilize pH, especially in combination with monopotassium phosphate or phosphoric acid.
- Analytical Reagent
- Employed in volumetric analysis, spectrophotometry, and as a component in media preparations.
Secondary Applications
- Culture Media – Used in microbial growth media to provide phosphates and potassium ions.
- Reagent-Grade Preparations – Assists in ion chromatography or as a base solution for chemical titrations.
- Water Chemistry Studies – Used in ion exchange and phosphate removal experiments.
KEY ATTRIBUTES
- Basic Identification Attributes
- Chemical Name: Dipotassium Hydrogen Phosphate
- Other Names: Potassium phosphate dibasic, Dipotassium phosphate
- Chemical Formula: Kâ‚‚HPOâ‚„
- CAS Number: 7758-11-4
- Grade: Extra Pure (Laboratory Grade)
- Molecular Weight: 174.18 g/mol
- Appearance: White crystalline powder or granules
- Odor: Odorless
2. Physical & Chemical Properties
- Melting Point: ~240 °C (decomposes)
- Solubility in Water: Soluble (149 g/L at 25 °C)
- pH (1% solution): ~8.6 – 9.6
- Density: ~2.44 g/cm³
- Hygroscopicity: Slightly hygroscopic
- Stability: Stable under normal conditions
3. Safety & Hazard Attributes
- GHS Classification: Not classified as hazardous
- Hazard Statements: None (generally considered low hazard)
- PPE Requirements:
- Lab coat
- Safety goggles
- Nitrile or latex gloves
- First Aid:
- Inhalation: Move to fresh air
- Skin Contact: Wash with soap and water
- Eye Contact: Rinse with plenty of water
- Ingestion: Rinse mouth, seek medical advice if unwell
4. Storage & Handling Attributes
- Storage Conditions:
- Store in a cool, dry, and well-ventilated area
- Keep container tightly closed
- Protect from moisture and incompatible materials (strong acids)
- Handling Notes:
- Avoid generating dust
- Handle with standard laboratory precautions
5. Regulatory & Compliance Attributes
- EC Number: 231-834-5
- UN Number: Not applicable (non-hazardous)
- REACH Registration: Registered
- DOT Classification (US): Not regulated
- Hazard Class (Transport): Not classified
6. Laboratory Applications
- Primary Uses:
- Buffering agent in biochemical and molecular biology labs
- Component of phosphate-buffered saline (PBS)
- Used in fertilizer research and nutrient media
- pH adjustment in laboratory experiments
- Other Uses:
- Food additive (when food-grade)
- Water treatment studies
- Reagent in pharmaceutical formulation R&D
SAFETY MEASURES
LABORATORY SAFETY & HANDLING
- PPE Required:
- Lab coat
- Gloves (nitrile or latex)
- Safety goggles
- Storage Guidelines:
- Store in a cool, dry, well-ventilated area
- Keep container tightly sealed
- Avoid moisture exposure (hygroscopic in nature)
- Handling Tips:
- Use clean, dry tools to avoid contamination
- Prepare solutions fresh when possible to maintain buffer integrity
FIRST AID MEASURES
- Inhalation: Move to fresh air; seek attention if irritation persists
- Skin Contact: Wash with plenty of water and soap
- Eye Contact: Rinse with water for 15 minutes and consult a physician
- Ingestion: Rinse mouth; not expected to be toxic in small amounts but seek medical advice
FIRE SAFETY MEASURES
- Flammability: Not flammable
- Firefighting Media: Water spray, foam, dry chemical, or COâ‚‚
- Decomposition Products: May release phosphorus oxides at high temperatures
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