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LABORATORY GRADE

EDTA Disodium Salt Extra Pure

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SHORT DESCRIPTION

EDTA Disodium Salt Extra Pure is a high-purity chelating agent widely used in laboratory settings for complexometric titrations, metal ion sequestration, and buffer preparation. It binds strongly with divalent and trivalent metal ions like calcium, magnesium, and iron, making it essential in water hardness testing, biochemical research, and analytical chemistry. Its stability and effectiveness in controlling metal ion activity also make it valuable in molecular biology protocols and diagnostic reagent formulations.

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Description

USES

Primary Laboratory Uses:

  1. Chelating agent in titrations (complexometric analysis):
     Commonly used to determine concentrations of metal ions like calcium, magnesium, iron, and lead in water hardness and metal assays.

  2. Buffer component in molecular biology and biochemistry:
     Helps stabilize pH and protect DNA and enzymes by binding divalent metal ions (e.g., Mg²⁺, Ca²⁺) that may catalyze degradation.

  3. Water softening in analytical procedures:
     Binds with metal ions in hard water to prevent interference with chemical reactions.

  4. Preservative in biological and chemical formulations:
     Inhibits metal-catalyzed oxidation and microbial growth.

Secondary Laboratory Uses:

  1. Electrophoresis buffer preparation (e.g., TAE, TBE):
     Used in DNA/RNA electrophoresis to maintain ionic strength and inhibit nuclease activity.

  2. Decalcification in histology and microscopy:
     Removes calcium from tissue samples for easier sectioning and analysis.

  3. Cleaning solution additive for laboratory glassware:
     Dissolves metal deposits and scales during glassware maintenance.

  4. Stabilizing agent in enzymatic reactions:
     Prevents unwanted metal-ion-mediated side reactions.

 

KEY PRODUCT FEATURES

KEY ATTRIBUTES

1. Basic Identification Attributes

  • Chemical Name: Ethylenediaminetetraacetic Acid Disodium Salt

  • Other Names: Disodium EDTA, EDTA-Na₂, Disodium ethylenediaminetetraacetate

  • Chemical Formula: C₁₀H₁₄N₂Na₂O₈·2H₂O (commonly as dihydrate)

  • CAS Number: 6381-92-6 (dihydrate), 139-33-3 (anhydrous)

  • Grade: Extra Pure (Laboratory Grade)

  • Molecular Weight: 372.24 g/mol (dihydrate)

  • Appearance: White crystalline powder

2. Physical & Chemical Properties

  • Solubility: Soluble in water (~10–100 g/L)

  • pH (1% solution): ~4–6

  • Melting Point: >240 °C (decomposes)

  • Stability: Stable under recommended storage conditions

  • Odor: Odorless

3. Safety & Hazard Attributes

  • GHS Classification: Not classified as hazardous under GHS

  • Hazard Statements:

    • May cause mild skin or eye irritation with prolonged exposure

  • PPE Requirements:

    • Safety goggles

    • Lab coat

    • Gloves

  • First Aid Measures:

    • Inhalation: Move to fresh air; seek medical attention if symptoms persist

    • Skin Contact: Wash with soap and water

    • Eye Contact: Rinse cautiously with water for several minutes

    • Ingestion: Rinse mouth; seek medical advice if feeling unwell

4. Storage & Handling Attributes

  • Storage Conditions:

    • Store in a tightly sealed container

    • Keep in a cool, dry, well-ventilated place

    • Protect from moisture and strong oxidizers

  • Handling Notes:

    • Avoid dust formation

    • Use in a fume hood if dust or aerosol is generated

5. Regulatory & Compliance Attributes

  • EC Number: 205-358-3

  • UN Number: Not regulated

  • Hazard Class: Non-hazardous

  • REACH Status: Registered

6. Laboratory Applications

  • Primary Uses:

    • Chelating agent for metal ions in analytical and biochemical applications

    • Used in complexometric titrations (especially Ca²⁺ and Mg²⁺)

    • Reagent in buffers, detergents, and DNA extraction protocols

  • Other Uses:

    • Water softening in lab experiments

    • Preparation of metal ion-free solutions

    • Preservative in some biological formulations

 

SAFETY HANDLING PRECAUTIONS

SAFETY MEASURES

SAFETY & HANDLING

  • PPE: Lab coat, gloves, and safety goggles recommended

  • Storage Conditions:

    • Store in a cool, dry place

    • Keep container tightly closed and away from moisture and incompatible substances

  • Incompatible With: Strong oxidizers, strong acids

STABILITY & REACTIVITY

  • Stability: Stable under standard lab conditions

  • Reactivity: Chelates metal ions strongly; not suitable for metal-ion dependent reactions

  • pH Range of Solutions: Typically 4–9 (commonly used around pH 7–8)

FIRST AID MEASURES

  • Inhalation: Move to fresh air; seek medical attention if symptoms persist

  • Skin Contact: Wash with soap and water

  • Eye Contact: Rinse with water for several minutes; consult medical help if irritation persists

  • Ingestion: Rinse mouth; seek medical advice

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