Folin Ciocalteus Phenol Reagent Extra Pure

KEY ATTRIBUTES

1. Basic Identification Attributes

• Product Name: Folin-Ciocalteu’s Phenol Reagent

• Type: Colorimetric reagent

• Grade: Extra Pure (Laboratory Reagent Grade)

• Appearance: Clear to light yellow or amber-colored liquid

• Odor: Slight acidic or phenolic

2. Composition

A complex mixture, typically containing:

• Phosphomolybdic acid

• Phosphotungstic acid

• Hydrochloric acid or sulfuric acid (stabilizing agents)

• Lithium sulfate or other buffering salts

• Water

3. Physical & Chemical Properties

• Solubility: Soluble in water

• pH: Strongly acidic

• Reactivity: Reacts with phenolic and polyphenolic compounds to form blue-colored complexes

• Stability: Stable under normal lab conditions when sealed and stored properly

• Storage Conditions:
  • Store in tightly sealed amber bottles

  • Keep in a cool, dry place away from light

4. Safety & Hazard Attributes

• Hazard Classification: Corrosive (due to acidic components)

• GHS Symbols: ⚠️ or ☣️ depending on concentration

• Hazard Statements:
  • H314: Causes severe skin burns and eye damage

  • H302: Harmful if swallowed

  • H335: May cause respiratory irritation

• PPE Requirements:
  • Lab coat

  • Nitrile or chemical-resistant gloves

  • Safety goggles or face shield

  • Use in well-ventilated area or fume hood

• First Aid Measures:
  • Skin Contact: Rinse with water for at least 15 minutes

  • Eye Contact: Rinse cautiously with water; remove contacts if present

  • Inhalation: Move to fresh air

  • Ingestion: Do not induce vomiting; rinse mouth and seek immediate medical help

5. Storage & Handling Attributes

• Storage Notes:
  • Protect from heat and sunlight

  • Store away from oxidizing or reducing agents

  • Label clearly and keep capped tightly

• Handling Notes:
  • Avoid inhalation of fumes

  • Do not pipette by mouth

  • Use clean, dry glassware for dispensing

6. Regulatory & Compliance Attributes

• CAS Number: [Not standardized as it’s a mixture]

• Regulatory Status:
  • Not classified as a hazardous material for transport in small lab quantities

  • May require reporting under local chemical safety regulations

7. Laboratory Applications

• Primary Use:
  • Quantitative determination of total phenolic content in biological and food samples

  • Commonly used in antioxidant activity assays

• Secondary Uses:
  • Analysis of polyphenols in plant extracts

  • Teaching tool in colorimetric and spectrophotometric analysis techniques

  • Validation of food and beverage quality