Methylene Blue Powder Extra Pure

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Methylene Blue Powder Extra Pure is a high-grade dye with diverse laboratory and industrial applications. It is widely used as a biological stain in microscopy for highlighting cellular structures such as nuclei, bacteria, and parasites, particularly in blood smear and bacteriological studies. In chemistry, it serves as a redox indicator due to its distinct color change properties, and in aquaculture, it acts as a treatment for fungal and bacterial infections in fish. Its extra pure formulation ensures maximum staining clarity, minimal contamination, and reliable results in research, diagnostics, and educational settings.

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Categories: Analytical Reagents, Biochemical Reagents, LABORATORY CHEMICALS, microbiology-and-cell-culture-reagents Tags: AnalyticalReagent, BiochemicalReagent, CellStaining, ExtraPure, LaboratoryStain, MethyleneBlue, MicrobiologyReagents, microscopystain, RedoxIndicator, SpecialtyLabChemicals

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Table of Contents

Methylene Blue Powder Extra Pure

Primary Uses:

Biological Stain – Widely used for staining animal and bacterial cells (e.g., in microbiology and histology) to highlight cellular structures such as nuclei and cytoplasm.
Redox Indicator – Serves as an oxidation-reduction indicator in various analytical chemistry applications.

Secondary Uses:

Aquaculture & Fish Medication – Used to treat fungal and bacterial infections in aquarium and hatchery settings.
Research Reagent – Applied in studies involving mitochondrial respiration, membrane permeability, and nerve tissue viability.
Dye in Microscopy Slides – Helps visualize cell morphology in prepared slide specimens.

PRODUCT KEY FEATURES

1. Basic Identification Attributes

Chemical Name: Methylene Blue
Synonyms: Basic Blue 9, Methylthioninium chloride
Chemical Formula: C₁₆H₁₈ClN₃S
CAS Number: 61-73-4
Molecular Weight: 319.85 g/mol
Appearance: Dark greenish-blue crystalline powder
Odor: Odorless
Solubility: Soluble in water and ethanol (forms deep blue solution)
Grade: Extra Pure

2. Safety & Hazard Attributes

GHS Classification:
- Eye Irritant (Category 2A)
- Skin Irritant (Category 2)
- Acute Toxicity (Oral, Category 4 — in large quantities)
Hazard Statements:
- H302: Harmful if swallowed
- H315: Causes skin irritation
- H319: Causes serious eye irritation
Precautionary Statements:
- P264: Wash hands thoroughly after handling
- P280: Wear protective gloves/eye protection
- P305+P351+P338: IF IN EYES: Rinse cautiously with water
PPE Required:
- Gloves
- Lab coat
- Safety goggles or face shield
First Aid:
- Eye Contact: Rinse cautiously with water
- Skin Contact: Wash with soap and water
- Inhalation: Remove to fresh air
- Ingestion: Rinse mouth and seek medical advice
Fire Hazards:
- Not highly flammable
- Use water spray, CO₂, dry chemical for fire extinguishing

3. Storage & Handling

Storage Conditions:
- Store in a tightly sealed container
- Keep in a cool, dry, and dark location
- Avoid moisture and strong oxidizing agents
Handling Tips:
- Avoid dust formation
- Prevent skin or clothing contact—stains persistently

4. Laboratory Applications

Primary Uses:
- Biological stain for microscopy (nuclei and bacteria visualization)
- Indicator in redox titrations
- Redox dye in electron transport chain studies
Secondary Uses:
- Aquaculture and aquarium antiseptic
- Diagnostic reagent in medical and microbiological labs
- Photodynamic therapy research and oxidative stress studies

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):

Wear a lab coat, nitrile gloves, and chemical safety goggles.
Use a dust mask or work in a fume hood to avoid inhalation of fine powder.

Handling:

Avoid contact with skin, eyes, and clothing.
Prevent formation and inhalation of dust.
Avoid ingestion.
Wash hands thoroughly after handling.

Storage:

Store in a tightly sealed container in a cool, dry, and well-ventilated area.
Protect from moisture, light, and heat.
Keep away from incompatible substances such as strong oxidizers and acids.

FIRST AID MEASURES

Inhalation:

Move the person to fresh air immediately.
Seek medical attention if symptoms like coughing or difficulty breathing occur.

Skin Contact:

Wash affected areas thoroughly with soap and water.
Remove contaminated clothing.
Get medical advice if irritation or staining persists.

Eye Contact:

Rinse eyes gently with plenty of water for at least 15 minutes.
Remove contact lenses if present and easy to do.
Seek medical attention.

Ingestion:

Rinse mouth with water.
Do not induce vomiting.
Seek medical advice promptly.

FIRE FIGHTING MEASURES

Flammability:

Not highly flammable but may burn if involved in a fire.

Extinguishing Media:

Use dry chemicals, CO₂, or foam.
Water spray can be used for cooling containers but may spread the powder.

Hazardous Combustion Products:

May emit carbon oxides, sulfur oxides, and nitrogen oxides when decomposed.

Firefighter Protection:

Use self-contained breathing apparatus (SCBA) and full protective gear.