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Rhodamine B

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Rhodamine B is a bright pink to reddish-purple synthetic dye belonging to the xanthene family. It is widely used as a fluorescent tracer dye and coloring agent due to its strong fluorescence and high photostability. Rhodamine B finds applications in analytical chemistry, biological staining, and industrial processes requiring vivid coloration or fluorescence detection.

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Description

 

Primary Uses

Analytical Chemistry and Fluorescence Tracing
Used as a fluorescent tracer dye in water flow studies, leak detection, and dye tracing in environmental research.

Applied in fluorescence microscopy and flow cytometry for staining and detecting biological samples.

Textile Industry
Utilized as a dye for coloring synthetic fibers, nylon, and polyester fabrics.

Provides bright and durable coloration in fabric dyeing processes.

Biological and Medical Research
Employed as a staining agent for cells and tissues in histology and cytology.

Used in fluorescence-based assays and imaging techniques to visualize biological structures.

 

Secondary Uses

Ink and Paint Manufacturing
Incorporated in inks and paints to produce vivid fluorescent colors.

Laser Dyes and Optics
Used in laser technology as a gain medium in dye lasers.

Plastics and Polymers
Added to plastics and resins to impart fluorescent properties for identification or decoration.

PRODUCT KEY FEATURES

 

Basic Identification Attributes
Chemical Name (IUPAC): [9-(2-carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]diethylammonium chloride

Common/Trade Name: Rhodamine B

CAS Number: 81-88-9

HS Code: 3204.17

Synonyms: Basic Violet 10, C.I. 45170

 

Physical & Chemical Properties
Physical State: Powder or solid crystals

Color & Odor: Dark pink to reddish-purple; odorless

Solubility: Soluble in water and ethanol

Melting Point: Approx. 228°C (decomposes)

Fluorescence: Strong pink-red fluorescence under UV light

 

Safety & Hazard Attributes
GHS Classification: Harmful if swallowed, suspected carcinogen

Toxicity: Moderate toxicity; handle with care

Exposure Limits: Use with appropriate safety measures

 

Storage & Handling Attributes
Storage Conditions: Store in tightly closed containers, away from light and moisture

Container Type: Sealed plastic or glass containers

Shelf Life: Stable if stored properly in a cool, dry place

Handling Precautions: Avoid inhalation and skin contact; use protective equipment

 

Regulatory & Compliance Attributes
Subject to chemical safety regulations

Use restricted or regulated in some countries due to toxicity concerns

 

Environmental & Health Impact
Biodegradability: Not readily biodegradable

Ecotoxicity: Toxic to aquatic organisms

Bioaccumulation: Potential for bioaccumulation

SAFETY HANDLING PRECAUTIONS

PPE Required: Gloves, goggles, protective clothing, and dust mask or respirator

Handling Guidelines: Use in well-ventilated areas; avoid dust generation and direct contact

Storage Measures: Keep containers tightly closed and stored in a dry environment away from light

 

First Aid Measures
Inhalation: Move to fresh air; seek medical attention if respiratory irritation occurs

Skin Contact: Wash with soap and water; seek medical advice if irritation persists

Eye Contact: Rinse with water for at least 15 minutes; seek medical care if irritation continues

Ingestion: Rinse mouth; do not induce vomiting; seek immediate medical help

 

Firefighting Measures
Fire Hazards: Combustible solid

Extinguishing Media: Use water spray, foam, dry chemical, or CO₂

Special Precautions: Wear full protective gear and self-contained breathing apparatus if involved in fire

Hazardous Combustion Products: Carbon oxides, nitrogen oxides

 

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