Trypsin Extra Pure

Name: Trypsin Extra Pure - Amaris Chemical Solutions
Price: 18.16 USD
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Trypsin Extra Pure is a high-purity, proteolytic enzyme derived from porcine pancreas, commonly used for its ability to hydrolyze peptide bonds in proteins at the carboxyl side of lysine and arginine residues. Supplied as a white to off-white lyophilized powder, Trypsin Extra Pure is ideal for laboratory applications requiring precise protein digestion, including cell culture detachment, protein sequencing, and enzymatic hydrolysis. Its extra pure grade ensures minimal contamination from other enzymes or impurities, making it suitable for sensitive biochemical and molecular biology workflows.

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Categories: Analytical Reagents, Biochemical Reagents Tags: BiochemicalReagent, CellCulture, EnzymeReagent, MolecularBiology, PharmaceuticalEnzyme, Protease, ProteinDigestion, Trypsin

Description

Table of Contents

Trypsin Extra Pure

PRIMARY USES

Analytical Reagent & Laboratory Applications
Widely used proteolytic enzyme for digestion of proteins in biochemical, molecular biology, and proteomics research
• Used to detach adherent cells from culture vessels in cell biology and tissue culture labs
• Essential in enzymatic digestion protocols for peptide mapping, protein sequencing, and mass spectrometry sample preparation
• Applied in protein modification and cleavage studies
• Key reagent in tissue dissociation for cell isolation and organotypic cultures
Pharmaceutical & Diagnostic Applications
Component in enzyme formulations for wound debridement and treatment of burns
• Used in digestive enzyme supplements and enzyme replacement therapies
• Utilized in diagnostic kits requiring proteolytic processing of samples
• Employed in pharmaceutical manufacturing for protein processing and modification

SECONDARY USES

Food Industry
Employed in hydrolysis of proteins to produce protein hydrolysates and bioactive peptides used as flavor enhancers and nutritional supplements
• Used for processing of animal-based protein products to improve digestibility
Cosmetic Industry
Incorporated into exfoliating and skin renewal formulations due to its protein digestion capability
• Used in anti-aging and skin peeling products to promote skin texture improvement
Biotechnological Research
Utilized in recombinant protein production workflows
• Employed in cell line development and scale-up processes for cell detachment

KEY PRODUCT FEATURES

1. Basic Identification

Chemical Name: Trypsin (from bovine pancreas or porcine sources)
CAS Number: 9002-07-7
HS Code: 3507.90.00
Molecular Formula: Protein enzyme (variable, dependent on source)
Synonyms: Protease, Pancreatic Trypsin, Proteolytic Enzyme, Trypsin USP Grade

2. Physical & Chemical Properties

Physical State: Off-white to pale yellow fine powder
Odor: Mild characteristic proteinaceous odor
Melting Point: Not applicable (denatures upon heating)
Density: Variable; powder bulk density approx. 0.3-0.6 g/cm³
Solubility: Soluble in aqueous buffers, especially at pH 7–9
pH Stability: Active in pH range 7–9; inactive in strongly acidic or alkaline conditions
Activity: Protease activity measured in USP units, BAEE units, or casein digestion units
Flash Point: Not applicable
Vapor Pressure: Not applicable

3. Safety & Hazard Attributes

GHS Classification: Skin sensitization (Category 1), Respiratory sensitization (Category 1)
NFPA Ratings: Health - 2 (due to allergenic potential), Flammability - 0, Reactivity - 0
Exposure Limits: Avoid inhalation; no established OSHA PEL but use dust control measures
Reactivity: Stable under recommended storage; denatures at high temperature and extreme pH
Allergen: Potential respiratory and skin sensitizer—handle with care

4. Storage & Handling

Store in a cool (2–8 °C), dry place protected from light and moisture
Keep container tightly closed to prevent contamination and moisture uptake
Use personal protective equipment (gloves, lab coat, goggles) and dust mask or respirator when handling powder
Avoid exposure to heat, moisture, and protease inhibitors
Shelf Life: Typically 1–2 years under recommended conditions

5. Regulatory & Compliance

Complies with USP standards for pharmaceutical grade enzymes
Approved for use in pharmaceutical, diagnostic, and food-grade applications (where applicable)
Not classified as hazardous for transport
Listed in major chemical inventories such as TSCA and REACH

6. Environmental & Health Impact

Biodegradable protein enzyme with minimal environmental persistence
Low ecotoxicity but may cause allergic reactions upon repeated exposure
Safe disposal recommended as per local environmental regulations

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Wear chemical-resistant gloves, lab coat, and safety goggles during handling
Use appropriate respiratory protection (dust mask or respirator) to avoid inhaling powder
Handle in a well-ventilated area or fume hood
Avoid skin and eye contact
Wash hands and exposed skin thoroughly after handling
Avoid creating dust clouds

FIRST AID MEASURES

Inhalation: Move to fresh air immediately; seek medical attention if respiratory irritation or allergic symptoms develop
Skin Contact: Wash affected area with soap and water; remove contaminated clothing; seek medical advice if irritation or rash develops
Eye Contact: Rinse immediately with plenty of water for at least 15 minutes; seek medical attention if irritation persists
Ingestion: Rinse mouth; do not induce vomiting; seek medical attention if symptoms appear

FIRE FIGHTING MEASURES

Non-flammable and non-combustible
Use appropriate extinguishing media (water spray, foam, dry chemical, CO₂) for surrounding fire
Firefighters should wear protective gear and self-contained breathing apparatus (SCBA) if exposed to dust or decomposition products
Thermal decomposition may release nitrogen oxides and other toxic fumes