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Disodium Hydrogen Phosphate Extra Pure
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SHORT DESCRIPTION
Disodium Hydrogen Phosphate Extra Pure is a high-purity, anhydrous inorganic salt widely used in laboratory settings as a buffering agent, pH regulator, and reagent in chemical analysis. It plays a crucial role in preparing phosphate buffer systems for biochemical assays, molecular biology protocols, and microbial culture media. With its reliable solubility and consistent reactivity, it supports accurate calibration of pH in solutions. Its extra pure grade ensures minimal contamination, making it ideal for use in sensitive analytical and research environments. Proper storage in airtight containers is recommended to prevent moisture absorption and preserve its effectiveness.
Description
USES
Primary Laboratory Uses:
- Buffer solution preparation (especially in phosphate buffer systems):
Essential in preparing phosphate buffers for biochemistry, molecular biology, and analytical chemistry applications. - Reagent in analytical chemistry:
Used in assays, titrations, and chemical analysis procedures. - Component of phosphate-buffered saline (PBS):
Combined with sodium dihydrogen phosphate to create isotonic solutions for maintaining biological specimen stability. - pH control and stabilization:
Employed in aqueous systems to maintain a constant pH environment. - Culture media preparation:
Used as a buffering agent in microbiological media to support microbial growth.
Secondary Laboratory Uses:
- Teaching and educational demonstrations:
Commonly included in lab experiments involving acid-base equilibria and buffer capacity. - Standardizing chemical solutions:
Sometimes used in standardizing acids and bases due to its buffering properties. - Research in water softening and treatment processes:
Investigated in simulated lab environments for its phosphate content and ability to prevent scale formation. - Ionic strength adjustment in biochemical reactions:
Helps create suitable conditions for enzyme activity or protein behavior studies.
KEY PRODUCT FEATURES
KEY ATTRIBUTES
1. Basic Identification Attributes
- Chemical Name: Disodium Hydrogen Phosphate
- Other Names: Disodium phosphate, Sodium phosphate dibasic
- Chemical Formula: Na₂HPO₄
- CAS Number: 7558-79-4 (anhydrous)
- Grade: Extra Pure (Laboratory Grade)
- Molecular Weight: 141.96 g/mol (anhydrous)
- Appearance: White crystalline or granular powder
- Odor: Odorless
2. Physical & Chemical Properties
- Melting Point: ~250 °C (decomposes)
- Solubility in Water: Freely soluble
- pH (1% solution): ~8.8 – 9.2
- Density: ~1.7 g/cm³
- Hygroscopicity: Hygroscopic
- Stability: Stable under normal lab conditions
3. Safety & Hazard Attributes
- GHS Classification: Not classified as hazardous
- Hazard Statements: None
- PPE Requirements:
- Lab coat
- Nitrile gloves
- Eye protection (safety goggles)
- First Aid Measures:
- Inhalation: Move to fresh air
- Skin Contact: Wash with water
- Eye Contact: Rinse thoroughly with water
- Ingestion: Rinse mouth; seek medical advice if necessary
4. Storage & Handling Attributes
- Storage Conditions:
- Keep container tightly closed
- Store in a cool, dry place
- Protect from moisture
- Handling Notes:
- Avoid inhaling dust
- Practice good laboratory hygiene
- Avoid contact with strong acids
5. Regulatory & Compliance Attributes
- EC Number: 231-448-7
- REACH Status: Registered
- UN Number: Not regulated
- DOT/ADR Classification: Not regulated
- Hazard Class (Transport): Non-hazardous
6. Laboratory Applications
- Primary Uses:
- Component in phosphate buffer systems
- pH control in chemical and biological solutions
- Reagent in analytical chemistry
- Use in molecular biology and biochemistry
- Other Uses:
- Water treatment studies
- Food-grade research (not for direct consumption in this grade)
- Educational demonstrations and buffer preparation
SAFETY HANDLING PRECAUTIONS
SAFETY MEASURES
LABORATORY SAFETY & HANDLING
- PPE Required:
- Safety goggles
- Lab coat
- Protective gloves
- Storage Recommendations:
- Store in a tightly sealed container
- Keep in a dry, well-ventilated area
- Avoid contact with moisture and acids
- Handling Precautions:
- Avoid generating dust
- Wash hands thoroughly after handling
FIRST AID MEASURES
- Inhalation: Move to fresh air; seek attention if symptoms develop
- Skin Contact: Wash with soap and water
- Eye Contact: Rinse cautiously with water for several minutes
- Ingestion: Rinse mouth; do not induce vomiting unless directed by medical personnel
FIRE & REACTIVITY INFORMATION
- Flammability: Non-flammable
- Incompatible Materials: Strong acids (may release heat and form phosphoric acid)
- Thermal Decomposition: May produce sodium oxides and phosphorus oxides
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