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Disodium Hydrogen Phosphate Dihydrate Extra Pure
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SHORT DESCRIPTION
Disodium Hydrogen Phosphate Dihydrate Extra Pure is a laboratory-grade chemical widely utilized as a buffering agent and pH stabilizer in analytical chemistry, molecular biology, and biochemical research. It is commonly used in the preparation of phosphate buffer solutions, supporting the maintenance of optimal conditions for enzyme activity and cell culture. This dihydrate form ensures accurate hydration levels for reliable solution formulation. Its high purity makes it suitable for sensitive lab applications requiring minimal interference from impurities. The compound should be kept tightly sealed in a dry environment to preserve its integrity and reactivity.
Description
USES
Primary Laboratory Uses:
- Buffer preparation (especially in phosphate buffer systems):
Widely used to maintain pH stability in biochemical and molecular biology experiments. - Reagent in analytical chemistry:
Used in qualitative and quantitative assays, including colorimetric and titration methods. - Component in saline and buffer solutions for biological research:
Often used in combination with sodium dihydrogen phosphate to create PBS (Phosphate Buffered Saline) for maintaining cell integrity. - pH adjustment in chemical reactions:
Employed to fine-tune pH in various aqueous solutions. - Media preparation in microbiology:
Acts as a buffering agent in culture media to support microbial growth.
Secondary Laboratory Uses:
- Standardizing solutions in titration procedures:
Used as a secondary standard in volumetric analysis. - Water treatment research:
Studied in laboratory-scale experiments for its role in corrosion control and precipitation reactions. - Educational experiments:
Common in teaching labs for demonstrating buffer systems and salt hydrolysis. - Protein precipitation or stabilization studies:
Occasionally used in protein chemistry experiments to influence ionic strength.
KEY PRODUCT FEATURES
KEY ATTRIBUTES
1. Basic Identification Attributes
- Chemical Name: Disodium Hydrogen Phosphate Dihydrate
- Other Names: Disodium phosphate dihydrate, Sodium phosphate dibasic dihydrate
- Chemical Formula: Na₂HPO₄·2H₂O
- CAS Number: 10028-24-7
- Grade: Extra Pure (Laboratory Grade)
- Molecular Weight: 177.99 g/mol
- Appearance: White crystalline powder
- Odor: Odorless
2. Physical & Chemical Properties
- Melting Point: Decomposes on heating
- Solubility in Water: Soluble (∼7.7 g/100 mL at 20 °C)
- pH (1% solution): ~8.0 – 9.5
- Density: ~1.52 g/cm³
- Hygroscopicity: Slightly hygroscopic
- Stability: Stable under normal lab conditions
3. Safety & Hazard Attributes
- GHS Classification: Not classified as hazardous
- Hazard Statements: None
- PPE Requirements:
- Laboratory coat
- Protective gloves (nitrile recommended)
- Safety goggles
- First Aid Measures:
- Inhalation: Move to fresh air; seek medical attention if symptoms occur
- Skin Contact: Rinse thoroughly with water
- Eye Contact: Flush eyes with water for several minutes
- Ingestion: Rinse mouth; consult a physician if unwell
4. Storage & Handling Attributes
- Storage Conditions:
- Store in a cool, dry, well-ventilated area
- Keep container tightly sealed
- Avoid moisture exposure
- Handling Notes:
- Avoid dust formation
- Use appropriate lab ventilation
- Follow standard laboratory hygiene practices
5. Regulatory & Compliance Attributes
- EC Number: 231-448-7
- REACH Status: Registered
- UN Number: Not applicable
- DOT/ADR Classification: Not regulated
- Hazard Class (Transport): Not classified
6. Laboratory Applications
- Primary Uses:
- Preparation of phosphate buffer systems
- pH control in chemical and biological solutions
- Reagent in biochemical and analytical chemistry
- Use in cell culture and molecular biology applications
- Other Uses:
- Component in some cleaning agents and water softeners (research use)
- Analytical reagent in food and beverage testing
SAFETY HANDLING PRECAUTIONS
SAFETY MEASURES
LABORATORY SAFETY & HANDLING
- PPE Required:
- Lab coat
- Safety goggles
- Nitrile or latex gloves
- Storage Guidelines:
- Store in a cool, dry location
- Keep container tightly closed and away from moisture
- Avoid prolonged exposure to air to prevent hydration changes
- Handling Tips:
- Handle under dry conditions
- Use clean utensils to avoid contamination
FIRST AID MEASURES
- Inhalation: Move to fresh air; seek medical attention if symptoms occur
- Skin Contact: Wash thoroughly with water and soap
- Eye Contact: Flush eyes with water for at least 15 minutes
- Ingestion: Rinse mouth and seek medical attention if large quantities are consumed
FIRE SAFETY MEASURES
- Flammability: Not flammable
- Extinguishing Media: Use water spray, dry chemical, foam, or COâ‚‚
- Decomposition Products: May produce sodium oxides and phosphorus oxides under intense heat
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