Leishman Stainpowder Extra Pure

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Leishman Stain Powder Extra Pure is a high-quality, finely milled formulation used extensively in hematology and parasitology for the microscopic staining of blood smears. Comprising mainly methylene blue and eosin, this stain is essential for differentiating white and red blood cells, as well as detecting malarial parasites and other blood-borne pathogens. Its excellent staining clarity and consistency make it a staple in clinical laboratories, diagnostic centers, and research institutions. The powder is reconstituted in methanol before use and must be handled with care, avoiding inhalation or contact with skin and eyes. Proper storage in a dry, cool environment ensures long-term stability and performance.

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Categories: Analytical Reagents, LABORATORY CHEMICALS, microbiology-and-cell-culture-reagents Tags: AnalyticalChemicals, BloodStain, CellCultureStain, CytologicalStain, Hematology, LeishmanStain, MicrobiologyReagents, SpecialLabChemicals

Description

Leishman Stainpowder Extra Pure

Primary Laboratory Uses:

Hematology (Blood Smear Staining):
- Primarily used for staining blood smears to differentiate blood cell types, especially for observing white blood cells, red blood cells, and platelets under a microscope.
Parasitology (Malaria Diagnosis):
- Extensively used for diagnosing malaria by staining Plasmodium parasites within red blood cells.
Cytological Staining:
- Applied in cytological preparations to identify and study nuclear and cytoplasmic morphology of cells.

Secondary Laboratory Uses:

Veterinary Diagnostics:
- Utilized in veterinary labs for hematological and parasitic studies in animal blood samples.
Training and Education:
- Frequently used in medical, clinical, and microbiology education for demonstrating cell morphology and staining techniques.
Quality Control Testing:
- In clinical laboratories, used to verify staining protocols and standardize microscopy diagnostics across batches.

KEY PRODUCT FEATURES

1. Basic Identification Attributes

Chemical Name: Leishman Stain Powder
Synonyms: Leishman's Stain, Leishman Powder
Composition: A dry mixture primarily of Methylene Blue and Eosin, often in methanol-soluble form
Appearance: Dark violet to bluish-purple powder
Solubility: Soluble in methanol or ethanol
Grade: Extra Pure (suitable for laboratory diagnostics and microscopy)

2. Safety & Hazard Attributes

GHS Classification:
- Acute toxicity – Category 4 (oral, inhalation)
- Eye irritation – Category 2A
- Carcinogenicity – Category 2 (due to methylene blue content)
Hazard Statements:
- H302: Harmful if swallowed
- H315: Causes skin irritation
- H319: Causes serious eye irritation
- H351: Suspected of causing cancer
Precautionary Statements:
- P261: Avoid breathing dust
- P280: Wear protective gloves, clothing, eye and face protection
- P301+P312: If swallowed, call a poison center or doctor
- P305+P351+P338: If in eyes, rinse cautiously with water
Personal Protective Equipment (PPE):
- Lab coat, gloves, safety goggles
- Dust mask or fume hood if handling in powder form
First Aid Measures:
- Inhalation: Move to fresh air, seek medical attention
- Skin Contact: Wash thoroughly with soap and water
- Eye Contact: Rinse with water for at least 15 minutes
- Ingestion: Rinse mouth; seek immediate medical advice
Fire Hazards:
- Not flammable, but combustible
- Emits toxic fumes under fire (carbon oxides, nitrogen oxides)
- Use dry chemical, CO₂, or alcohol-resistant foam to extinguish

3. Storage & Handling Attributes

Storage Conditions:
- Keep tightly closed in a cool, dry place
- Protect from light and moisture
- Store away from incompatible chemicals (oxidizers)
Handling Tips:
- Minimize dust generation
- Avoid contact with eyes, skin, and clothing
- Handle in well-ventilated areas

4. Laboratory Applications

Primary Uses:
- Hematology: Widely used for staining blood smears to identify blood parasites (e.g., Plasmodium in malaria) and differential white blood cell counts
- Microscopy: Staining nuclei and cytoplasm of blood cells for enhanced contrast under light microscopy
Secondary Uses:
- Teaching labs for demonstrating blood smear techniques
- Veterinary diagnostics
- Staining bone marrow and other tissue smears for cellular differentiation

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):

Wear a lab coat, nitrile gloves, and protective goggles.
Use a dust mask or work in a fume hood to avoid inhalation of fine powders or fumes.

Handling:

Handle with care to avoid inhalation, skin contact, or eye exposure.
Avoid formation of dust and prevent spills.
Wash hands thoroughly after use.

Storage:

Store in a cool, dry, and well-ventilated area.
Keep the container tightly closed and away from direct light.
Do not store near oxidizing agents or acids.

FIRST AID MEASURES

Inhalation:

Move the person to fresh air immediately.
Seek medical attention if symptoms such as coughing or dizziness occur.

Skin Contact:

Rinse affected areas with plenty of water and soap.
Remove contaminated clothing.
Get medical help if irritation or discoloration persists.

Eye Contact:

Rinse cautiously with clean water for 15 minutes.
Remove contact lenses if easy to do.
Seek medical attention immediately.

Ingestion:

Do not induce vomiting.
Rinse mouth with water.
Get urgent medical attention.

FIRE FIGHTING MEASURES

Flammability:

May be combustible at high temperatures. Avoid creating dust clouds near ignition sources.

Extinguishing Media:

Use dry chemical, carbon dioxide (CO₂), or alcohol-resistant foam.
Water spray may be used to cool containers but might spread powdered material.

Hazardous Combustion Products:

May emit carbon oxides, nitrogen oxides, and other toxic fumes when burned.

Firefighter Protection:

Wear self-contained breathing apparatus (SCBA) and full fire-resistant protective gear.
Avoid inhalation of combustion products.