Rhodamine B

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Rhodamine B is a bright pink to reddish-purple synthetic dye belonging to the xanthene family. It is widely used as a fluorescent tracer dye and coloring agent due to its strong fluorescence and high photostability. Rhodamine B finds applications in analytical chemistry, biological staining, and industrial processes requiring vivid coloration or fluorescence detection.

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Category: Dyes and Pigments Tags: Basic Violet 10, Fluorescent Dye, Rhodamine B, Textile Dyeing & Printing, Water treatment

Description

Table of Contents

Rhodamine B

Primary Uses

Analytical Chemistry and Fluorescence Tracing

Used as a fluorescent tracer dye in water flow studies, leak detection, and dye tracing in environmental research.
Applied in fluorescence microscopy and flow cytometry for staining and detecting biological samples.

Textile Industry

Utilized as a dye for coloring synthetic fibers, nylon, and polyester fabrics.
Provides bright and durable coloration in fabric dyeing processes.

Biological and Medical Research

Employed as a staining agent for cells and tissues in histology and cytology.
Used in fluorescence-based assays and imaging techniques to visualize biological structures.

Secondary Uses

Ink and Paint Manufacturing

Incorporated in inks and paints to produce vivid fluorescent colors.

Laser Dyes and Optics

Used in laser technology as a gain medium in dye lasers.

Plastics and Polymers

Added to plastics and resins to impart fluorescent properties for identification or decoration.

KEY PRODUCT FEATURES

1. Basic Identification Attributes

Chemical Name (IUPAC): [9-(2-carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]diethylammonium chloride
Common/Trade Name: Rhodamine B
CAS Number: 81-88-9
HS Code: 3204.17
Synonyms: Basic Violet 10, C.I. 45170

2. Physical & Chemical Properties

Physical State: Powder or solid crystals
Color & Odor: Dark pink to reddish-purple; odorless
Solubility: Soluble in water and ethanol
Melting Point: Approx. 228°C (decomposes)
Fluorescence: Strong pink-red fluorescence under UV light

3. Safety & Hazard Attributes

GHS Classification: Harmful if swallowed, suspected carcinogen
Toxicity: Moderate toxicity; handle with care
Exposure Limits: Use with appropriate safety measures

4. Storage & Handling Attributes

Storage Conditions: Store in tightly closed containers, away from light and moisture
Container Type: Sealed plastic or glass containers
Shelf Life: Stable if stored properly in a cool, dry place
Handling Precautions: Avoid inhalation and skin contact; use protective equipment

5. Regulatory & Compliance Attributes

Subject to chemical safety regulations
Use restricted or regulated in some countries due to toxicity concerns

6. Environmental & Health Impact

Biodegradability: Not readily biodegradable
Ecotoxicity: Toxic to aquatic organisms
Bioaccumulation: Potential for bioaccumulation

SAFETY HANDLING PRECAUTIONS

Safety Handling Precautions

PPE Required: Gloves, goggles, protective clothing, and dust mask or respirator
Handling Guidelines: Use in well-ventilated areas; avoid dust generation and direct contact
Storage Measures: Keep containers tightly closed and stored in a dry environment away from light

First Aid Measures

Inhalation: Move to fresh air; seek medical attention if respiratory irritation occurs
Skin Contact: Wash with soap and water; seek medical advice if irritation persists
Eye Contact: Rinse with water for at least 15 minutes; seek medical care if irritation continues
Ingestion: Rinse mouth; do not induce vomiting; seek immediate medical help

Firefighting Measures

Fire Hazards: Combustible solid
Extinguishing Media: Use water spray, foam, dry chemical, or CO₂
Special Precautions: Wear full protective gear and self-contained breathing apparatus if involved in fire
Hazardous Combustion Products: Carbon oxides, nitrogen oxides