Ascorbic Acid Extra Pure

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SHORT DESCRIPTION

Ascorbic Acid Extra Pure, commonly known as Vitamin C, is a high-purity, white to slightly yellow crystalline powder widely used in laboratory settings for analytical, biochemical, and pharmaceutical research. It functions as a potent reducing agent and antioxidant, making it valuable in redox titrations, radical scavenging assays, and studies of oxidative stress and enzyme activity. Ascorbic acid is also used in food chemistry experiments and as a stabilizer in formulations prone to oxidation. The extra pure grade ensures minimal contaminants, supporting precise and reproducible results in sensitive applications. It should be stored in tightly sealed containers, protected from light, heat, and moisture to preserve its stability and effectiveness.

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Category: LABORATORY CHEMICALS Tags: AnalyticalGrade, AntioxidantActivity, BiochemicalReagent, FreeRadicalScavenger, HighPurityAscorbicAcid, RedoxTitration, ReducingAgent, VitaminC

Description

USES

Primary Uses

Reducing Agent in Redox Titrations
- Acts as a mild and selective reducing agent, especially in iodometric titrations, where it reduces iodine (I₂) to iodide (I⁻).
Antioxidant in Biochemical and Food Chemistry Studies
- Commonly used to prevent oxidation of sensitive molecules like polyphenols, enzymes, and fats in research on degradation and stability.
Standard for Vitamin C Quantification Assays
- Serves as a reference compound in DCPIP titrations, spectrophotometric assays, and nutritional analysis of foods.
Model Compound in Redox Kinetics and Mechanism Studies
- Ideal for teaching and research on reaction kinetics, electron transfer, and radical scavenging.
Buffer and pH Modifier in Biochemical Solutions
- In low concentrations, it can help maintain mildly acidic pH and support enzyme activity in biological experiments.

Secondary Uses

Preservative in Sample Preparation and Storage
- Added to aqueous or biological samples to prevent oxidative degradation during storage or analysis.
Reagent in Plant and Tissue Culture Media
- Protects cells from oxidative stress, and stimulates growth in some plant tissue culture formulations.
Chelating Agent for Metal Ions
- Chelates Fe³⁺ and other metal ions, sometimes used in metal transport, uptake, or redox balance studies.
Control Compound in Antioxidant Screening
- Frequently used as a benchmark antioxidant when evaluating radical scavenging activity of other substances (e.g., DPPH assay).
Demonstration of Non-Enzymatic Browning Inhibition
- Employed in food chemistry teaching labs to study Maillard reaction suppression or delay in fruit/vegetable oxidation.

KEY PRODUCT FEATURES

KEY ATTRIBUTES

1. Basic Identification Attributes

Chemical Name: Ascorbic Acid
CAS Number: 50-81-7
HS Code: 29362700 (Vitamins and their derivatives – Ascorbic acid and its salts)
Molecular Formula: C₆H₈O₆
Synonyms:
- Vitamin C
- L-Ascorbic acid
- 3-Keto-L-gulofuranolactone
- L-threo-hex-2-enono-1,4-lactone

2. Physical & Chemical Properties

Physical State: Solid (crystalline powder)
Color & Odor: White to slightly yellow; odorless with slightly acidic taste
Boiling Point: Decomposes before boiling
Melting Point: ~190–192 °C (with decomposition)
Density/Specific Gravity: ~1.65 g/cm³
Solubility:
- Water: Freely soluble (~330 g/L at 20 °C)
- Alcohol: Slightly soluble
pH Level: ~2.1–2.6 (in 5% aqueous solution)
Vapor Pressure: Negligible
Flash Point: Not flammable
Autoignition Temperature: Not applicable
Viscosity: Not applicable

3. Safety & Hazard Attributes

Hazard Class (GHS):
- Not classified as hazardous under GHS
- May cause mild eye or skin irritation in sensitive individuals
NFPA Ratings:
- Health: 0
- Flammability: 0
- Reactivity: 0
Exposure Limits:
- No established OSHA or ACGIH limits
Reactivity:
- Sensitive to air, heat, and light (oxidizes readily)
- Incompatible with strong oxidizing agents and alkalis

4. Storage & Handling Attributes

Storage Conditions:
- Store in airtight container, in a cool, dry, dark place
- Protect from light and moisture
Incompatible Materials:
- Strong oxidizers (e.g., chlorine, nitrates)
- Bases (promote degradation)
Container Type:
- Amber glass or opaque plastic
Shelf Life & Expiration Date:
- ~2 years if stored properly
Special Handling Requirements:
- Use gloves and avoid exposure to air and moisture during use

5. Regulatory & Compliance Attributes

Regulatory Status:
- Listed under TSCA, REACH, GRAS (Generally Recognized as Safe for food)
Hazard Symbols (GHS Pictograms):
- None required for non-industrial handling
Transportation Restrictions:
- Not regulated as hazardous
Waste Disposal Method:
- Small amounts can be flushed with water
- Bulk waste: dispose via certified waste facility

6. Environmental & Health Impact

Ecotoxicity:
- Low toxicity; biodegradable
Persistence in Environment:
- Degrades rapidly in presence of light and oxygen
Carcinogenicity/Mutagenicity:
- Not classified as carcinogenic or mutagenic
Biodegradability:
- Readily biodegradable

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):
- Wear lab coat, safety goggles, and nitrile gloves.
- Use in a well-ventilated area or under a fume hood if handling large quantities or powders.
Handling:
- Avoid inhalation of dust and contact with eyes or skin.
- Handle with care to minimize airborne particles.
- Avoid contamination with oxidizing agents or bases—it may degrade the compound.
Storage:
- Store in a cool, dry, well-ventilated area.
- Keep container tightly sealed, protected from light, moisture, and air (to prevent oxidation).
- Use amber or opaque containers if possible.
Hygiene Measures:
- Wash hands thoroughly after handling.
- Avoid eating, drinking, or smoking while using the chemical.
- Clean up any spilled powder immediately using appropriate PPE.

FIRST AID MEASURES

Inhalation:
- Remove person to fresh air.
- Seek medical advice if respiratory irritation occurs (coughing, sneezing, sore throat).
Skin Contact:
- Wash skin with plenty of water and soap.
- Remove contaminated clothing.
- Seek medical attention if irritation or rash develops.
Eye Contact:
- Rinse immediately with plenty of clean water for at least 15 minutes.
- Lift eyelids to ensure full flushing.
- Seek medical attention if irritation persists.
Ingestion:
- Rinse mouth with water.
- Do not induce vomiting.
- Generally considered low toxicity, but seek medical advice if large amounts are ingested or if symptoms occur (diarrhea, cramps).

FIRE FIGHTING MEASURES

Suitable Extinguishing Media:
- Use dry chemicals, CO₂, foam, or water spray.
Specific Hazards:
- Ascorbic acid is combustible, though not highly flammable.
- Thermal decomposition can produce:
  - Carbon monoxide (CO)
  - Carbon dioxide (CO₂)
  - Irritating organic fumes
Protective Equipment:
- Firefighters should wear SCBA (self-contained breathing apparatus) and protective gear.
Firefighting Instructions:
- Cool containers with water spray.
- Avoid breathing smoke or vapors.
- Prevent runoff—though not highly toxic, ascorbic acid may cause pH changes in water bodies.