Bicarbonate Indicator Extra Pure

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Bicarbonate Indicator Extra Pure is a high-purity, specially formulated solution used in laboratory settings to detect and monitor changes in bicarbonate ion concentration, particularly in biological and environmental experiments. It typically contains a pH-sensitive dye that changes color in response to shifts in carbon dioxide or bicarbonate levels, making it valuable in studies of respiration, photosynthesis, and water quality. This indicator is often employed in teaching labs to visually demonstrate gas exchange or acid-base balance. The extra pure grade ensures consistent performance, color clarity, and minimal background interference in precise analytical work. It should be stored in a cool, dark place in a tightly closed container to maintain its sensitivity and stability.

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Categories: Analytical Reagents, microbiology-and-cell-culture-reagents, Spectroscopy Reagents Tags: AnalyticalReagent, BicarbonateIndicator, CO2Detection, EnvironmentalChemistry, HighPurityChemical, LabGradeIndicator, PHIndicator, SpectroscopyReagent

Description

USES

Table of Contents

Primary Uses

Indicator for CO₂ Concentration in Aqueous Systems
- Used to visually monitor changes in dissolved carbon dioxide, particularly in photosynthesis and respiration experiments.
Teaching Photosynthesis in Plant Physiology Labs
- Demonstrates the uptake of CO₂ by aquatic plants (e.g., Elodea) through color change as bicarbonate ions are consumed during carbon fixation.
Buffer System Visualization
- Illustrates pH shifts in bicarbonate–carbonic acid equilibrium in teaching acid–base chemistry and buffering action.
Qualitative CO₂ Detection
- Acts as a colorimetric detector in lab setups for identifying CO₂ evolution during fermentation or respiration studies.

Secondary Uses

Classroom Demonstrations
- Ideal for visual experiments showing acid–base reactions, gas exchange, or carbonate buffering, often in educational institutions.
Color Indicator in Field CO₂ Measurement Kits
- May be used in portable lab kits or environmental sampling tools for quick CO₂ assessment in water or soil solutions.
Calibration of pH-Responsive Systems

Useful for calibrating pH sensors or colorimetric assays involving weak acid–base systems.

Product Key Features

KEY ATTRIBUTES

1. Basic Identification Attributes

Chemical Name: Bicarbonate Indicator (Mixture – typically includes bromocresol green or phenol red in a suitable buffer)
Common Name: Bicarbonate Indicator
CAS Number: Mixture (individual components like Bromocresol Green: 76-60-8, Phenol Red: 143-74-8)
HS Code: 3822.00.00 (Diagnostic or laboratory reagents)
Molecular Formula: Not applicable (Mixture)
Synonyms:
- Bicarbonate pH indicator
- CO₂ indicator solution
- Alkalinity indicator solution
- Carbon dioxide detection reagent

2. Physical & Chemical Properties

Physical State: Liquid (aqueous solution)
Color & Odor: Green to yellow color shift (if Bromocresol Green is used), odorless
Boiling Point: ~100 °C (aqueous base)
Melting Point: Not applicable
Density: ~1.0 g/cm³
Solubility:
- Water: Fully miscible
- Organic Solvents: Not typically used in organic media
pH Range (color change):
- Bromocresol Green: 3.8 (yellow) – 5.4 (blue)
- Phenol Red: 6.4 (yellow) – 8.2 (red)
Vapor Pressure: Like water (~24 mmHg at 25 °C)
Flash Point: Not flammable
Autoignition Temperature: Not applicable
Viscosity: Similar to water

3. Safety & Hazard Attributes

GHS Classification:
- Not hazardous under normal laboratory use
NFPA Ratings:
- Health: 1
- Flammability: 0
- Reactivity: 0
Exposure Limits:
- No OSHA/ACGIH limits established for diluted indicators
Reactivity:
- Stable under normal conditions
- Avoid strong acids or bases which may affect indicator accuracy

Storage & Handling Attributes

Storage Conditions:
- Store at room temperature, away from light
- Avoid contamination and extreme pH exposure
Incompatible Materials:
- Strong acids and alkalis
- Oxidizing agents
Container Type:
- Amber glass or HDPE bottle
Shelf Life:
- 1–2 years if stored properly
Special Handling Requirements:
- Use gloves, avoid skin contact
- Use in well-ventilated area or under fume hood when working with concentrated acids

5. Regulatory & Compliance Attributes

Regulatory Status:
- Not regulated as hazardous under GHS, OSHA, or REACH
Hazard Symbols (GHS pictograms):
- None typically required
Transportation Restrictions:
- Not classified as hazardous for transport
Waste Disposal Method:
- Dispose as non-hazardous lab waste in accordance with institutional/local guidelines

6. Environmental & Health Impact

Ecotoxicity:
- Low at laboratory-use concentrations
Persistence in Environment:
- Readily biodegradable (due to organic dye components)
Carcinogenicity/Mutagenicity:
- No known carcinogenic or mutagenic effects at lab concentrations
Biodegradability:
- Components are generally biodegradable

Safety Handling Precautions

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):
- Wear lab coat, protective gloves (nitrile recommended), and safety goggles.
- Use in a well-ventilated space or under a fume hood if in liquid form or aerosolized.
Handling:
- Handle with care to avoid spillage or aerosol formation.
- Avoid direct contact with skin or eyes.
- Do not breathe in dust, vapor, or mist.
Storage:
- Keep container tightly sealed.
- Store in a cool, dry place, protected from light and incompatible materials (strong oxidizers or acids).
Hygiene Measures:
- Wash hands thoroughly after handling.
- Do not eat, drink, or smoke during use.

FIRST AID MEASURES

Inhalation:
- Move the affected person to fresh air.
- Seek medical attention if discomfort or symptoms persist.
Skin Contact:
- Rinse skin with plenty of water and soap.
- Remove contaminated clothing.
Eye Contact:
- Rinse cautiously with water for at least 15 minutes.
- Seek medical assistance if irritation persists.
Ingestion:
- Rinse mouth.
- Do not induce vomiting.
- Seek immediate medical advice.

FIRE FIGHTING MEASURES

Extinguishing Media:
- Use carbon dioxide, dry chemical powder, foam, or water spray.
Fire Hazards:
- May emit toxic or irritating fumes if heated or decomposed (e.g., carbon oxides depending on the composition).
- Not highly flammable but may support combustion of other substances.
Protective Equipment:
- Firefighters should wear self-contained breathing apparatus (SCBA) and appropriate fire gear.