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LABORATORY GRADE

Brilliant Green Extra Pure

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Brilliant Green Extra Pure is a high-purity, intense green dye primarily used in microbiology and analytical laboratories for staining and selective microbial inhibition. It is especially effective in culture media to isolate specific bacterial strains, such as Salmonella spp., by suppressing the growth of gram-positive organisms. In addition to its antimicrobial role, it serves as a histological stain in biological research and is used in tracing, titration, and other colorimetric assays. The extra pure grade guarantees high dye content, minimal impurities, and excellent consistency, ensuring reliable results in sensitive and precise laboratory work. It should be stored in a tightly sealed container, away from direct light and heat, to preserve its stability and effectiveness.

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Description

Primary Uses
Microbiological Selective Agent

Used as a selective dye in culture media like Brilliant Green Agar to inhibit gram-positive bacteria while promoting gram-negative bacterial growth, particularly Salmonella species.

Biological Stain

Employed as a cytoplasmic stain or counterstain in histology and microbiology, particularly in staining fungi, spores, or cell structures.

pH Indicator and Dye

Functions as a colorimetric indicator in some analytical titrations, especially where a distinct green shift is desired upon pH change.

Secondary Uses
Model Compound in Dye Kinetics Studies

Used in adsorption, diffusion, and photodegradation experiments, especially in environmental chemistry to simulate dye pollution.

Demonstration of Spectroscopic Behavior

Utilized in UV-Vis absorbance experiments due to its strong chromophore, helping students understand molar absorptivity and dye spectra.

Toxicity Bioassays

Sometimes used in bioassays or aquatic toxicology labs to evaluate dye toxicity in model organisms, such as Daphnia or algae.

PRODUCT KEY FEATURES

Basic Identification Attributes
Chemical Name: Brilliant Green

Other Names: Malachite Green G, Basic Green 1

CAS Number: 633-03-4

HS Code: 3204.13.00

Molecular Formula: C₂₇H₃₄N₂·Cl

Grade: Extra Pure (laboratory use)
2. Physical & Chemical Properties
Physical State: Crystalline powder

Color & Odor: Green; odorless

Molecular Weight: 385.04 g/mol

Melting Point: ~210°C (decomposes)

Solubility in Water: Soluble (forms a bright green solution)

Solubility in Alcohol: Soluble

pH of Aqueous Solution: ~4–6

Light Sensitivity: Decomposes upon prolonged exposure

Staining Properties: Strong dye; adheres to organic materials and tissues

3. Safety & Hazard Attributes
GHS Classification:

Acute Toxicity (Oral, Category 4)

Eye Irritation (Category 2A)

GHS Pictograms: ⚠️

NFPA Ratings:

Health: 2

Flammability: 1

Reactivity: 0

Exposure Limits: Not established (handle with caution)

PPE Requirements:

Lab coat, gloves, safety goggles

Inhalation/Ingestion Risks:

Harmful if swallowed or inhaled

Skin/Eye Contact:

May cause irritation; avoid direct contact

4. Storage & Handling Attributes
Storage Conditions:

Store in a tightly closed container in a cool, dry place

Protect from light and moisture

Incompatible Materials:

Strong oxidizers, acids

Container Type: Amber glass or HDPE

Shelf Life: 2–3 years (if properly stored)

Special Handling Requirements:

Handle in well-ventilated area; avoid dust formation
5. Regulatory & Compliance Attributes
Regulatory Status:

Not approved for food or drug use

May be regulated in some jurisdictions due to toxicity

UN Number: Not regulated for transport

Waste Disposal Method:

Dispose of as hazardous chemical waste via licensed facility

Avoid release to drains or environment
6. Environmental & Health Impact
Ecotoxicity:

Toxic to aquatic life; persistent in water systems

Persistence in Environment:

High; not readily biodegradable

Carcinogenicity/Mutagenicity:

Suspected mutagen; long-term exposure risks under study

Health Concerns:

Avoid ingestion or chronic exposure; not for clinical use

SAFETY HANDLING PRECAUTIONS

Personal Protective Equipment (PPE):

Wear lab coat, safety goggles, and nitrile gloves.

Use a dust mask or respirator if working with powder or aerosol.

Handling:

Handle in a fume hood or well-ventilated area.

Avoid skin and eye contact; avoid inhalation of dust or vapors.

Prevent formation of dust.

Storage:

Store in a cool, dry, and well-ventilated area.

Keep container tightly closed and protected from light.

Store away from oxidizing agents and acids.

Incompatibilities:

Strong oxidizing agents, strong acids, and light (causes degradation).

FIRST AID MEASURES
Inhalation:

Move victim to fresh air.

If breathing is difficult, provide oxygen.

Seek medical attention if symptoms persist.

Skin Contact:

Wash immediately with soap and water.

Remove contaminated clothing and wash before reuse.

Seek medical help if irritation develops.

Eye Contact:

Rinse cautiously with water for at least 15 minutes.

Remove contact lenses if present and easy to do.

Get medical attention immediately.

Ingestion:

Rinse mouth with water. Do not induce vomiting.

Seek immediate medical attention.

May cause irritation of the digestive tract.

FIRE FIGHTING MEASURES
Extinguishing Media:

Use CO₂, dry chemical powder, alcohol-resistant foam, or water spray.

Hazardous Combustion Products:

May release carbon oxides, nitrogen oxides, and toxic fumes when burned.

Protective Equipment for Firefighters:

Wear self-contained breathing apparatus (SCBA) and full protective suit.

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