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Bromelain Extra Pure
Original price was: $ 18.00.$ 17.34Current price is: $ 17.34.
Bromelain Extra Pure is a high-purity proteolytic enzyme derived from pineapple, widely used in laboratory research involving protein digestion, enzymatic reactions, and biochemical assays. It is particularly valued for its ability to hydrolyze a broad range of protein substrates, making it useful in studies of protein structure, enzyme kinetics, and tissue dissociation. In pharmaceutical and biomedical laboratories, it is also investigated for its anti-inflammatory, fibrinolytic, and mucolytic properties. The extra pure grade ensures minimal contamination from other enzymes or plant materials, offering consistent and reliable enzymatic activity. Bromelain should be stored in a cool, dry place in tightly sealed containers to maintain its stability and effectiveness.
Bromelain Extra Pure
Primary Uses
- Proteolytic Enzyme Studies
- Used in biochemistry labs to study proteolysis, enzyme kinetics, and protein substrate breakdown, as Bromelain hydrolyzes proteins into peptides and amino acids.
- Tissue Dissociation for Cell Culture
- Applied in cell biology for gentle dissociation of tissues to isolate viable cells, especially where preservation of surface antigens is critical.
- Anti-inflammatory Mechanism Research
- Employed in pharmacology labs to study anti-inflammatory pathways, especially its effects on prostaglandins, bradykinin, and fibrin.
- Allergenicity and Stability Testing
- Utilized in toxicology and allergy studies to evaluate enzymatic allergens and their stability under different conditions.
Secondary Uses
- Model Enzyme in Educational Labs
- Frequently used in teaching labs to demonstrate enzyme activity, temperature/pH dependence, and enzyme inhibition.
- Clarification in Bioprocess Simulation
- Simulates industrial enzymatic treatments, such as in juice clarification, useful for food technology and biotechnology experiments.
- Electrophoresis Sample Prep
- Occasionally used to degrade proteins in samples for preparatory steps before gel electrophoresis or other downstream analysis.
| PACK SIZE | 500 grams Plastic Tin |
|---|
1. Basic Identification Attributes
- Chemical Name: Bromelain
- CAS Number: 9001-00-7
- HS Code: 3507.90.90
- Molecular Formula: Not applicable (complex mixture of enzymes)
- Synonyms: Ananase, Pineapple extract enzyme, Bromelin
- Grade: Extra Pure (for laboratory/research use)
2. Physical & Chemical Properties
- Physical State: Powder
- Color & Odor: Off-white to light yellow, slight characteristic odor
- Solubility in Water: Soluble
- Solubility in Alcohol: Insoluble
- Optimum pH Range: 6.0–7.0
- Isoelectric Point (pI): ~4.6
- Temperature Sensitivity: Denatures above 60°C
- Enzymatic Activity: Measured in GDU/g or MCU/g depending on source
- Boiling/Melting Point: Not applicable (decomposes)
- Stability: Stable under dry, cool storage; loses activity with heat/humidity
3. Safety & Hazard Attributes
- GHS Classification:
- Respiratory Sensitizer (Category 1)
- Skin Sensitizer (Category 1)
- GHS Pictograms: ⚠️
- NFPA Ratings:
- Health: 2
- Flammability: 0
- Reactivity: 0
- Exposure Limits: Not established (avoid inhalation of enzyme dust)
- Inhalation/Ingestion Risks:
- May cause allergic reactions or respiratory irritation
- Skin/Eye Contact:
- May cause irritation or sensitization
4. Storage & Handling Attributes
- Storage Conditions:
- Store in tightly sealed container at 2–8°C
- Protect from moisture and light
- Incompatible Materials:
- Oxidizing agents, acids
- Container Type: Airtight plastic or glass containers
- Shelf Life: 1–2 years under optimal storage
- Special Handling Requirements:
- Use gloves, dust mask, and eye protection in lab
- Work in fume hood if powder handling is frequent
5. Regulatory & Compliance Attributes
- Regulatory Status:
- Not hazardous under normal lab use
- Not intended for therapeutic or food use in this grade
- Hazard Symbols: ⚠️
- Transportation Restrictions: Not regulated for transport
- Waste Disposal Method:
- Dispose of as non-hazardous organic waste
- Avoid environmental release
6. Environmental & Health Impact
- Ecotoxicity:
- Low environmental hazard; biodegradable
- Persistence in Environment:
- Not persistent; degrades in moist environments
- Carcinogenicity/Mutagenicity:
- Not classified as carcinogenic
- Health Concerns:
- Potential allergen via inhalation or skin exposure
SAFETY PRECAUTIONS
- Personal Protective Equipment (PPE):
- Wear lab coat, nitrile gloves, and safety goggles.
- If handling powders, use a dust mask or particulate respirator.
- Handling:
- Avoid generating dust; handle in a fume hood or well-ventilated area.
- Avoid contact with skin, eyes, and clothing.
- Prevent inhalation or ingestion of the enzyme.
- Storage:
- Store in a cool (2–8 °C), dry, and light-protected location.
- Keep the container tightly sealed.
- Avoid exposure to heat and moisture which can degrade enzymatic activity.
- Incompatibilities:
- Strong oxidizers, acids, and bases may denature the enzyme.
FIRST AID MEASURES
- Inhalation:
- Remove victim to fresh air.
- Seek medical attention if breathing becomes difficult or if allergy symptoms occur.
- Skin Contact:
- Wash skin with plenty of water and soap.
- Remove contaminated clothing.
- Seek medical advice if irritation or allergic reaction develops.
- Eye Contact:
- Rinse cautiously with water for several minutes.
- Remove contact lenses if present and easy to do.
- Continue rinsing and seek medical help if irritation persists.
- Ingestion:
- Rinse mouth with water.
- Do not induce vomiting.
- Get medical attention if large amounts are swallowed or if symptoms occur.
FIRE FIGHTING MEASURES
- Extinguishing Media:
- Use water spray, CO₂, foam, or dry chemical powder.
- Hazardous Combustion Products:
- May emit carbon monoxide (CO) and carbon dioxide (CO₂) upon decomposition.
- Protective Equipment for Firefighters:
- Firefighters should wear self-contained breathing apparatus (SCBA) and protective clothing.
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