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Bromelain Extra Pure Amaris Chemicals
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Bromine Liquid Extra Pure Original price was: $ 17.00.Current price is: $ 16.78.
LABORATORY GRADE

Bromelain Extra Pure

Original price was: $ 18.00.Current price is: $ 17.34.

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Bromelain Extra Pure is a high-purity proteolytic enzyme derived from pineapple, widely used in laboratory research involving protein digestion, enzymatic reactions, and biochemical assays. It is particularly valued for its ability to hydrolyze a broad range of protein substrates, making it useful in studies of protein structure, enzyme kinetics, and tissue dissociation. In pharmaceutical and biomedical laboratories, it is also investigated for its anti-inflammatory, fibrinolytic, and mucolytic properties. The extra pure grade ensures minimal contamination from other enzymes or plant materials, offering consistent and reliable enzymatic activity. Bromelain should be stored in a cool, dry place in tightly sealed containers to maintain its stability and effectiveness.

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Description

Bromelain Extra Pure

Primary Uses

  1. Proteolytic Enzyme Studies
    • Used in biochemistry labs to study proteolysis, enzyme kinetics, and protein substrate breakdown, as Bromelain hydrolyzes proteins into peptides and amino acids.

  2. Tissue Dissociation for Cell Culture
    • Applied in cell biology for gentle dissociation of tissues to isolate viable cells, especially where preservation of surface antigens is critical.

  3. Anti-inflammatory Mechanism Research
    • Employed in pharmacology labs to study anti-inflammatory pathways, especially its effects on prostaglandins, bradykinin, and fibrin.

  4. Allergenicity and Stability Testing
    • Utilized in toxicology and allergy studies to evaluate enzymatic allergens and their stability under different conditions.

Secondary Uses

  1. Model Enzyme in Educational Labs
    • Frequently used in teaching labs to demonstrate enzyme activity, temperature/pH dependence, and enzyme inhibition.

  2. Clarification in Bioprocess Simulation
    • Simulates industrial enzymatic treatments, such as in juice clarification, useful for food technology and biotechnology experiments.

  3. Electrophoresis Sample Prep
    • Occasionally used to degrade proteins in samples for preparatory steps before gel electrophoresis or other downstream analysis.
KEY PRODUCT FEATURES

1. Basic Identification Attributes

  • Chemical Name: Bromelain
  • CAS Number: 9001-00-7
  • HS Code: 3507.90.90
  • Molecular Formula: Not applicable (complex mixture of enzymes)
  • Synonyms: Ananase, Pineapple extract enzyme, Bromelin
  • Grade: Extra Pure (for laboratory/research use)

2. Physical & Chemical Properties

  • Physical State: Powder
  • Color & Odor: Off-white to light yellow, slight characteristic odor
  • Solubility in Water: Soluble
  • Solubility in Alcohol: Insoluble
  • Optimum pH Range: 6.0–7.0
  • Isoelectric Point (pI): ~4.6
  • Temperature Sensitivity: Denatures above 60°C
  • Enzymatic Activity: Measured in GDU/g or MCU/g depending on source
  • Boiling/Melting Point: Not applicable (decomposes)
  • Stability: Stable under dry, cool storage; loses activity with heat/humidity

3. Safety & Hazard Attributes 

  • GHS Classification:
    • Respiratory Sensitizer (Category 1)
    • Skin Sensitizer (Category 1)
  • GHS Pictograms: ⚠️
  • NFPA Ratings:
    • Health: 2
    • Flammability: 0
    • Reactivity: 0
  • Exposure Limits: Not established (avoid inhalation of enzyme dust)
  • Inhalation/Ingestion Risks:
    • May cause allergic reactions or respiratory irritation
  • Skin/Eye Contact:
    • May cause irritation or sensitization

4. Storage & Handling Attributes

  • Storage Conditions:
    • Store in tightly sealed container at 2–8°C
    • Protect from moisture and light
  • Incompatible Materials:
    • Oxidizing agents, acids
  • Container Type: Airtight plastic or glass containers
  • Shelf Life: 1–2 years under optimal storage
  • Special Handling Requirements:
    • Use gloves, dust mask, and eye protection in lab
    • Work in fume hood if powder handling is frequent

5. Regulatory & Compliance Attributes

  • Regulatory Status:
    • Not hazardous under normal lab use
    • Not intended for therapeutic or food use in this grade
  • Hazard Symbols: ⚠️
  • Transportation Restrictions: Not regulated for transport
  • Waste Disposal Method:
    • Dispose of as non-hazardous organic waste
    • Avoid environmental release

6. Environmental & Health Impact

  • Ecotoxicity:
    • Low environmental hazard; biodegradable
  • Persistence in Environment:
    • Not persistent; degrades in moist environments
  • Carcinogenicity/Mutagenicity:
    • Not classified as carcinogenic
  • Health Concerns:
    • Potential allergen via inhalation or skin exposure
SAFETY HANDLING PRECAUTIONS

 SAFETY PRECAUTIONS

  1. Personal Protective Equipment (PPE):
    • Wear lab coat, nitrile gloves, and safety goggles.
    • If handling powders, use a dust mask or particulate respirator.
  2. Handling:
    • Avoid generating dust; handle in a fume hood or well-ventilated area.
    • Avoid contact with skin, eyes, and clothing.
    • Prevent inhalation or ingestion of the enzyme.
  3. Storage:
    • Store in a cool (2–8 °C), dry, and light-protected location.
    • Keep the container tightly sealed.
    • Avoid exposure to heat and moisture which can degrade enzymatic activity.
  4. Incompatibilities:
    • Strong oxidizers, acids, and bases may denature the enzyme.

FIRST AID MEASURES

  1. Inhalation:
    • Remove victim to fresh air.
    • Seek medical attention if breathing becomes difficult or if allergy symptoms occur.
  2. Skin Contact:
    • Wash skin with plenty of water and soap.
    • Remove contaminated clothing.
    • Seek medical advice if irritation or allergic reaction develops.
  3. Eye Contact:
    • Rinse cautiously with water for several minutes.
    • Remove contact lenses if present and easy to do.
    • Continue rinsing and seek medical help if irritation persists.
  4. Ingestion:
    • Rinse mouth with water.
    • Do not induce vomiting.
    • Get medical attention if large amounts are swallowed or if symptoms occur.

FIRE FIGHTING MEASURES

  1. Extinguishing Media:
    • Use water spray, CO₂, foam, or dry chemical powder.
  2. Hazardous Combustion Products:
    • May emit carbon monoxide (CO) and carbon dioxide (CO₂) upon decomposition.
  3. Protective Equipment for Firefighters:
    • Firefighters should wear self-contained breathing apparatus (SCBA) and protective clothing.

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