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Brilliant Green Extra Pure
Original price was: $ 17.00.$ 16.67Current price is: $ 16.67.
Brilliant Green Extra Pure is a high-purity, intense green dye primarily used in microbiology and analytical laboratories for staining and selective microbial inhibition. It is especially effective in culture media to isolate specific bacterial strains, such as Salmonella spp., by suppressing the growth of gram-positive organisms. In addition to its antimicrobial role, it serves as a histological stain in biological research and is used in tracing, titration, and other colorimetric assays. The extra pure grade guarantees high dye content, minimal impurities, and excellent consistency, ensuring reliable results in sensitive and precise laboratory work. It should be stored in a tightly sealed container, away from direct light and heat, to preserve its stability and effectiveness.
Brilliant Green Extra Pure
Primary Uses
- Microbiological Selective Agent
- Used as a selective dye in culture media like Brilliant Green Agar to inhibit gram-positive bacteria while promoting gram-negative bacterial growth, particularly Salmonella species.
- Biological Stain
- Employed as a cytoplasmic stain or counterstain in histology and microbiology, particularly in staining fungi, spores, or cell structures.
- pH Indicator and Dye
- Functions as a colorimetric indicator in some analytical titrations, especially where a distinct green shift is desired upon pH change.
Secondary Uses
- Model Compound in Dye Kinetics Studies
- Used in adsorption, diffusion, and photodegradation experiments, especially in environmental chemistry to simulate dye pollution.
- Demonstration of Spectroscopic Behavior
- Utilized in UV-Vis absorbance experiments due to its strong chromophore, helping students understand molar absorptivity and dye spectra.
- Toxicity Bioassays
- Sometimes used in bioassays or aquatic toxicology labs to evaluate dye toxicity in model organisms, such as Daphnia or algae.
| PACK SIZE | 25 grams Plastic Tin |
|---|
1. Basic Identification Attributes
- Chemical Name: Brilliant Green
- Other Names: Malachite Green G, Basic Green 1
- CAS Number: 633-03-4
- HS Code: 3204.13.00
- Molecular Formula: C₂₇H₃₄N₂·Cl
- Grade: Extra Pure (laboratory use)
2. Physical & Chemical Properties
- Physical State: Crystalline powder
- Color & Odor: Green; odorless
- Molecular Weight: 385.04 g/mol
- Melting Point: ~210°C (decomposes)
- Solubility in Water: Soluble (forms a bright green solution)
- Solubility in Alcohol: Soluble
- pH of Aqueous Solution: ~4–6
- Light Sensitivity: Decomposes upon prolonged exposure
- Staining Properties: Strong dye; adheres to organic materials and tissues
3. Safety & Hazard Attributes
- GHS Classification:
- Acute Toxicity (Oral, Category 4)
- Eye Irritation (Category 2A)
- GHS Pictograms: ⚠️
- NFPA Ratings:
- Health: 2
- Flammability: 1
- Reactivity: 0
- Exposure Limits: Not established (handle with caution)
- PPE Requirements:
- Lab coat, gloves, safety goggles
- Inhalation/Ingestion Risks:
- Harmful if swallowed or inhaled
- Skin/Eye Contact:
- May cause irritation; avoid direct contact
4. Storage & Handling Attributes
- Storage Conditions:
- Store in a tightly closed container in a cool, dry place
- Protect from light and moisture
- Incompatible Materials:
- Strong oxidizers, acids
- Container Type: Amber glass or HDPE
- Shelf Life: 2–3 years (if properly stored)
- Special Handling Requirements:
- Handle in well-ventilated area; avoid dust formation
5. Regulatory & Compliance Attributes
- Regulatory Status:
- Not approved for food or drug use
- May be regulated in some jurisdictions due to toxicity
- UN Number: Not regulated for transport
- Waste Disposal Method:
- Dispose of as hazardous chemical waste via licensed facility
- Avoid release to drains or environment
6. Environmental & Health Impact
- Ecotoxicity:
- Toxic to aquatic life; persistent in water systems
- Persistence in Environment:
- High; not readily biodegradable
- Carcinogenicity/Mutagenicity:
- Suspected mutagen; long-term exposure risks under study
- Health Concerns:
- Avoid ingestion or chronic exposure; not for clinical use
SAFETY PRECAUTIONS
- Personal Protective Equipment (PPE):
- Wear lab coat, safety goggles, and nitrile gloves.
- Use a dust mask or respirator if working with powder or aerosol.
- Handling:
- Handle in a fume hood or well-ventilated area.
- Avoid skin and eye contact; avoid inhalation of dust or vapors.
- Prevent formation of dust.
- Storage:
- Store in a cool, dry, and well-ventilated area.
- Keep container tightly closed and protected from light.
- Store away from oxidizing agents and acids.
- Incompatibilities:
- Strong oxidizing agents, strong acids, and light (causes degradation).
FIRST AID MEASURES
- Inhalation:
- Move victim to fresh air.
- If breathing is difficult, provide oxygen.
- Seek medical attention if symptoms persist.
- Skin Contact:
- Wash immediately with soap and water.
- Remove contaminated clothing and wash before reuse.
- Seek medical help if irritation develops.
- Eye Contact:
- Rinse cautiously with water for at least 15 minutes.
- Remove contact lenses if present and easy to do.
- Get medical attention immediately.
- Ingestion:
- Rinse mouth with water. Do not induce vomiting.
- Seek immediate medical attention.
- May cause irritation of the digestive tract.
FIRE FIGHTING MEASURES
- Extinguishing Media:
- Use CO₂, dry chemical powder, alcohol-resistant foam, or water spray.
- Hazardous Combustion Products:
- May release carbon oxides, nitrogen oxides, and toxic fumes when burned.
- Protective Equipment for Firefighters:
- Wear self-contained breathing apparatus (SCBA) and full protective suit.
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