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Bromine Liquid Extra Pure
LABORATORY GRADE

Bromelain Extra Pure

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Bromelain Extra Pure is a high-purity proteolytic enzyme derived from pineapple, widely used in laboratory research involving protein digestion, enzymatic reactions, and biochemical assays. It is particularly valued for its ability to hydrolyze a broad range of protein substrates, making it useful in studies of protein structure, enzyme kinetics, and tissue dissociation. In pharmaceutical and biomedical laboratories, it is also investigated for its anti-inflammatory, fibrinolytic, and mucolytic properties. The extra pure grade ensures minimal contamination from other enzymes or plant materials, offering consistent and reliable enzymatic activity. Bromelain should be stored in a cool, dry place in tightly sealed containers to maintain its stability and effectiveness.

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Description

Primary Uses
Proteolytic Enzyme Studies

Used in biochemistry labs to study proteolysis, enzyme kinetics, and protein substrate breakdown, as Bromelain hydrolyzes proteins into peptides and amino acids.

Tissue Dissociation for Cell Culture

Applied in cell biology for gentle dissociation of tissues to isolate viable cells, especially where preservation of surface antigens is critical.

Anti-inflammatory Mechanism Research

Employed in pharmacology labs to study anti-inflammatory pathways, especially its effects on prostaglandins, bradykinin, and fibrin.

Allergenicity and Stability Testing

Utilized in toxicology and allergy studies to evaluate enzymatic allergens and their stability under different conditions.

Secondary Uses
Model Enzyme in Educational Labs

Frequently used in teaching labs to demonstrate enzyme activity, temperature/pH dependence, and enzyme inhibition.

Clarification in Bioprocess Simulation

Simulates industrial enzymatic treatments, such as in juice clarification, useful for food technology and biotechnology experiments.

Electrophoresis Sample Prep

Occasionally used to degrade proteins in samples for preparatory steps before gel electrophoresis or other downstream analysis.

PRODUCT KEY FEATURES

. Basic Identification Attributes
Chemical Name: Bromelain

CAS Number: 9001-00-7

HS Code: 3507.90.90

Molecular Formula: Not applicable (complex mixture of enzymes)

Synonyms: Ananase, Pineapple extract enzyme, Bromelin

Grade: Extra Pure (for laboratory/research use)
2. Physical & Chemical Properties
Physical State: Powder

Color & Odor: Off-white to light yellow, slight characteristic odor

Solubility in Water: Soluble

Solubility in Alcohol: Insoluble

Optimum pH Range: 6.0–7.0

Isoelectric Point (pI): ~4.6

Temperature Sensitivity: Denatures above 60°C

Enzymatic Activity: Measured in GDU/g or MCU/g depending on source

Boiling/Melting Point: Not applicable (decomposes)

Stability: Stable under dry, cool storage; loses activity with heat/humidity
3. Safety & Hazard Attributes
GHS Classification:

Respiratory Sensitizer (Category 1)

Skin Sensitizer (Category 1)

GHS Pictograms: ⚠️

NFPA Ratings:

Health: 2

Flammability: 0

Reactivity: 0

Exposure Limits: Not established (avoid inhalation of enzyme dust)

Inhalation/Ingestion Risks:

May cause allergic reactions or respiratory irritation

Skin/Eye Contact:

May cause irritation or sensitization
4. Storage & Handling Attributes
Storage Conditions:

Store in tightly sealed container at 2–8°C

Protect from moisture and light

Incompatible Materials:

Oxidizing agents, acids

Container Type: Airtight plastic or glass containers

Shelf Life: 1–2 years under optimal storage

Special Handling Requirements:

Use gloves, dust mask, and eye protection in lab

Work in fume hood if powder handling is frequent
5. Regulatory & Compliance Attributes
Regulatory Status:

Not hazardous under normal lab use

Not intended for therapeutic or food use in this grade

Hazard Symbols: ⚠️

Transportation Restrictions: Not regulated for transport

Waste Disposal Method:

Dispose of as non-hazardous organic waste

Avoid environmental release
6. Environmental & Health Impact
Ecotoxicity:

Low environmental hazard; biodegradable

Persistence in Environment:

Not persistent; degrades in moist environments

Carcinogenicity/Mutagenicity:

Not classified as carcinogenic

Health Concerns:

Potential allergen via inhalation or skin exposure

SAFETY HANDLING PRECAUTIONS

Personal Protective Equipment (PPE):

Wear lab coat, nitrile gloves, and safety goggles.

If handling powders, use a dust mask or particulate respirator.

Handling:

Avoid generating dust; handle in a fume hood or well-ventilated area.

Avoid contact with skin, eyes, and clothing.

Prevent inhalation or ingestion of the enzyme.

Storage:

Store in a cool (2–8 °C), dry, and light-protected location.

Keep the container tightly sealed.

Avoid exposure to heat and moisture which can degrade enzymatic activity.

Incompatibilities:

Strong oxidizers, acids, and bases may denature the enzyme.

FIRST AID MEASURES
Inhalation:

Remove victim to fresh air.

Seek medical attention if breathing becomes difficult or if allergy symptoms occur.

Skin Contact:

Wash skin with plenty of water and soap.

Remove contaminated clothing.

Seek medical advice if irritation or allergic reaction develops.

Eye Contact:

Rinse cautiously with water for several minutes.

Remove contact lenses if present and easy to do.

Continue rinsing and seek medical help if irritation persists.

Ingestion:

Rinse mouth with water.

Do not induce vomiting.

Get medical attention if large amounts are swallowed or if symptoms occur.

FIRE FIGHTING MEASURES
Extinguishing Media:

Use water spray, CO₂, foam, or dry chemical powder.

Hazardous Combustion Products:

May emit carbon monoxide (CO) and carbon dioxide (CO₂) upon decomposition.

Protective Equipment for Firefighters:

Firefighters should wear self-contained breathing apparatus (SCBA) and protective clothing.

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