Cresol Red Indicator Extra Pure

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SHORT DESCRIPTION

Cresol Red Indicator Extra Pure is a high-purity pH indicator dye widely used in laboratory titrations, buffer solutions, and biochemical assays. It exhibits a clear color change from yellow to red over the pH range of approximately 7.2 to 8.8, making it suitable for detecting slight pH shifts in weakly basic environments. Its stable performance and extra pure grade ensure consistent results in analytical chemistry, molecular biology, and spectrophotometric applications. Ideal for use in precise pH monitoring, Cresol Red should be handled with care and stored away from light and moisture to preserve its integrity.

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Category: LABORATORY CHEMICALS Tags: AnalyticalChemistry, BufferSystems, ColorimetricAssay, CresolRed, LabGradeChemical, PHIndicator, Spectroscopy, TitrationReagent

Description

USES

Table of Contents

Primary Uses

pH Indicator in Acid–Base Titrations
- Cresol Red is a sulfonphthalein dye used to indicate pH changes with a distinct color transition:
  - Red in acidic (pH < 1.2)
  - Yellow in weakly acidic to neutral (pH ~2–8.8)
  - Purple in basic (pH > 8.8)
- Used in strong acid–strong base titrations and buffer solution testing.
Biochemical Buffer Marker
- Frequently used in molecular biology as a tracking dye during gel electrophoresis to monitor sample migration.
Teaching Tool in pH and Buffer Systems
- Common in chemistry education labs to visually demonstrate pH changes, buffer capacity, and acid-base equilibria.
Colorimetric pH Monitoring in Microplates or Solutions
- Suitable for high-throughput pH monitoring in microplate-based biochemical or microbial assays.

Secondary Uses

Dye for Protein and Nucleic Acid Electrophoresis
- Sometimes added to loading buffers as a visible tracking marker in agarose or polyacrylamide gels.
Component in Diagnostic Kits
- Used in biochemical test kits where pH shifts signal enzymatic or metabolic activity.
Model Compound for Dye Degradation Studies
- Serves as a test dye in photodegradation, ozonation, or advanced oxidation studies to track environmental dye breakdown.
Visual Endpoint Marker in Buffer Prep or CO₂ Testing
- Applied in experiments where a quick visual confirmation of CO₂ absorption (via pH shift) is needed.

KEY PRODUCT FEATURES

KEY ATTRIBUTES

1. Basic Identification Attributes

Chemical Name: Cresol Red
CAS Number: 1733-12-6
Chemical Formula: C₂₁H₁₈O₅S
Molar Mass: 366.43 g/mol
Synonyms: o-Cresolsulfonephthalein, Acid Red 7, Indicator Red
Grade: Extra Pure (Analytical Reagent grade)

2. Physical & Chemical Properties

Appearance: Reddish to brown-violet powder
Odor: Odorless
Solubility:
- Soluble in ethanol and slightly soluble in water
pH Range (Indicator):
- Color change from yellow to red: pH 7.2 to 8.8
Melting Point: >220 °C (decomposes)

3. Safety & Hazard Attributes

GHS Classification:
- H302: Harmful if swallowed
- H319: Causes serious eye irritation
NFPA Ratings:
- Health: 2
- Flammability: 1
- Reactivity: 0
First Aid Measures:
- Inhalation: Move to fresh air; seek medical attention if symptoms persist
- Skin Contact: Wash thoroughly with soap and water
- Eye Contact: Rinse cautiously for several minutes with clean water
- Ingestion: Rinse mouth, seek medical advice

4. Storage & Handling Attributes

Storage Conditions:
- Store tightly sealed in a dry, cool, and dark location
- Protect from moisture and light
Handling Advice:
- Wear gloves, lab coat, and eye protection
- Avoid dust formation and inhalation

5. Regulatory & Compliance Attributes

EINECS Number: 217-168-8
REACH Status: Compliant
UN Number: Not classified as dangerous for transport in solid form

6. Applications in Laboratory

Primary Use:
- pH indicator in analytical chemistry and titrations
Other Uses:
- Buffer system pH monitoring
- Electrophoresis loading dye
- Enzyme or biochemical assays
- Histological staining (in some modified protocols)

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

PPE Required:
- Lab coat
- Nitrile gloves
- Safety goggles or face shield
Handling:
- Handle in a well-ventilated area
- Avoid inhalation of dust or contact with eyes and skin
- Weigh and prepare in designated indicator prep zones
Storage:
- Store in tightly sealed amber container
- Keep in a dry, cool place away from light and acids
- Avoid humidity to preserve quality

FIRST AID MEASURES

Inhalation:
- Move to fresh air
- Seek medical attention if irritation or dizziness occurs
Skin Contact:
- Wash thoroughly with soap and water
- Remove contaminated clothing
Eye Contact:
- Rinse immediately with water for at least 15 minutes
- Get medical attention if irritation persists
Ingestion:
- Rinse mouth
- Do not induce vomiting—seek immediate medical help

FIREFIGHTING MEASURES

Flammability: Not highly flammable
Explosion Risk: Low under normal conditions
Extinguishing Media:
- Use CO₂, dry powder, or foam
- Avoid direct water spray
Firefighter Protection:
- Use self-contained breathing apparatus