Dextrose Monohydrate Extra Pure
SHORT DESCRIPTION
Dextrose Monohydrate Extra Pure is a high-purity crystalline form of glucose containing one molecule of water. It is widely used in laboratory settings as a carbon source in microbial culture media, fermentation processes, and biochemical assays. Its precise composition and consistent solubility make it ideal for preparing nutrient solutions, buffers, and test reagents. In research and educational labs, it supports various metabolic and enzymatic studies. This extra pure grade ensures minimal impurities, providing reliable results in sensitive analytical and biological applications. Store in a tightly sealed container in a cool, dry place to preserve its quality and prevent clumping.
USES
Primary Uses
- Microbiology Media Component
- Serves as a carbon and energy source in culture media for bacteria, fungi, and yeast growth.
- Fermentation Experiments
- Common substrate for fermentation studies and metabolic pathway analysis, particularly in biochemistry labs.
- Biochemical Reagent
- Used in assays involving enzymes like glucose oxidase, or calorimetric glucose detection methods.
- Osmotic Balance in Solutions
- Added to buffers or solutions to maintain osmotic pressure, especially in cell-based assays or perfusion systems.
Secondary Uses
- Standard in Analytical Chemistry
- Used to calibrate or validate sugar quantification methods (e.g., Fehling’s, Benedict’s, DNS assays).
- Model Compound for Carbohydrate Studies
- Helpful in exploring reducing sugar behavior, Maillard reactions, or starch hydrolysis experiments.
- Cryoprotectant Component
- Part of cryopreservation mixtures to protect cells or enzymes during freezing.
KEY ATTRIBUTES
1. Basic Identification Attributes
- Chemical Name: Dextrose Monohydrate
- Other Names: Glucose Monohydrate, D-Glucose Monohydrate
- CAS Number: 14431-43-7
- Chemical Formula: C₆H₁₂O₆·H₂O
- Molar Mass: 198.17 g/mol
- Grade: Extra Pure (Laboratory Grade)
2. Physical & Chemical Properties
- Appearance: White, crystalline powder or granules
- Odor: Odorless
- Taste: Sweet
- Solubility: Freely soluble in water; slightly soluble in ethanol
- Melting Point: ~83 °C (decomposes)
- pH (5% solution): 4.0 – 6.5
- Hygroscopicity: Hygroscopic (absorbs moisture from air)
3. Safety & Hazard Attributes
- GHS Classification: Not classified as hazardous
- NFPA Ratings:
- Health: 0
- Flammability: 1
- Reactivity: 0
- First Aid Measures:
- Inhalation: Move to fresh air
- Skin Contact: Wash with soap and water
- Eye Contact: Rinse thoroughly with water
- Ingestion: Non-toxic in typical lab quantities; rinse mouth if needed
4. Storage & Handling Attributes
- Storage Conditions:
- Store in a cool, dry, and well-ventilated area
- Keep container tightly closed to prevent clumping due to moisture
- Use desiccator if necessary
- Handling Advice:
- Avoid inhalation of dust
- Use clean tools to avoid contamination
- Wash hands after handling
5. Regulatory & Compliance Attributes
- EINECS Number: 200-075-1
- UN Number: Not regulated
- REACH Status: Exempt (naturally occurring sugar)
6. Applications in Laboratory
- Primary Use:
- Used as a nutrient in microbiological media and fermentation
- Other Uses:
- Carbon source in cell culture and biochemical research
- Reducing sugar in Benedict’s and Fehling’s tests
- Analytical chemistry (as a standard sugar)
- Used in pharmaceutical formulations and reagent buffers
- Calibration of polarimeters
SAFETY PRECAUTIONS
- PPE:
- Lab coat
- Safety goggles
- Gloves (especially when handling in large quantities or preparing solutions)
- Handling Notes:
- Avoid creating dust—use in well-ventilated areas
- Keep away from moisture to maintain stability
- Storage Conditions:
- Store in a cool, dry place
- Keep container tightly sealed
- Hygroscopic – protect from humidity and direct sunlight
FIRST AID MEASURES
- Inhalation: Move to fresh air; seek medical help if irritation persists
- Skin Contact: Wash with water; no significant hazard
- Eye Contact: Rinse cautiously with water for several minutes
- Ingestion: Generally low toxicity, but lab-grade material should not be consumed
FIREFIGHTING MEASURES
- Flammability: Combustible as a powder
- Hazards: May form explosive dust-air mixtures
- Extinguishing Media: Water spray, foam, dry chemical, or CO₂
Special Notes: Avoid stirring up dust near open flames
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