Disodium Phosphate Extra Pure

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SHORT DESCRIPTION

Disodium Phosphate Extra Pure is a high-grade inorganic compound commonly used in laboratories as a buffering agent, emulsifier, and reagent. It is particularly valuable in preparing phosphate buffer solutions for biochemical and molecular biology applications. Its reliable pH-stabilizing properties make it ideal for use in analytical chemistry, food analysis, and preparation of reagent mixtures. With excellent solubility in water and minimal impurities, this extra pure form ensures accuracy and consistency in experimental results across a wide range of laboratory procedures.

Description

USES

Primary Laboratory Uses:

  1. Buffer preparation (especially phosphate buffer systems):
    A key component in creating stable buffer solutions for biochemical and analytical experiments.

  2. Reagent in analytical chemistry:
    Used in colorimetric analysis and other quantitative assays due to its buffering and ionic properties.

  3. pH control in laboratory solutions:
    Helps maintain neutral to slightly basic conditions during reactions or solution storage.

  4. Component of PBS (phosphate-buffered saline):
    Widely used in biological and molecular experiments to mimic physiological conditions.

  5. Nutrient in microbiological media:
    Supplies phosphate ions essential for microbial growth in culture formulations.

Secondary Laboratory Uses:

  1. Educational use in buffer and acid-base experiments:
    Ideal for demonstrating buffer action in teaching labs.

  2. Electrophoresis buffer component:
    Occasionally used in gel electrophoresis to maintain consistent pH.

  3. Water softening simulations and studies:
    Studied for its ability to chelate calcium and magnesium in lab-scale water treatment experiments.

  4. Stabilizing agent in enzyme reactions:
    Used to maintain optimal ionic strength and pH in enzymatic assays.

 

KEY PRODUCT FEATURES

KEY ATTRIBUTES

1. Basic Identification Attributes

  • Chemical Name: Disodium Phosphate

  • Other Names: Sodium phosphate dibasic, Disodium hydrogen phosphate

  • Chemical Formula: Na₂HPO₄

  • CAS Number:

    • Anhydrous: 7558-79-4

    • Dihydrate: 10028-24-7

    • Heptahydrate: 7782-85-6

  • Grade: Extra Pure (Laboratory Grade)

  • Molecular Weight:

    • 141.96 g/mol (anhydrous)

    • 177.99 g/mol (dihydrate)

  • Appearance: White crystalline powder or granules

  • Odor: Odorless

2. Physical & Chemical Properties

  • Solubility: Soluble in water

  • pH (1% solution): ~8.5 – 9.5

  • Melting Point: ~250 °C (decomposes)

  • Density: ~1.7 – 2.0 g/cm³ (varies with hydration)

  • Hygroscopic: Yes, especially in hydrated forms

3. Safety & Hazard Attributes

  • GHS Classification: Not classified as hazardous

  • Hazard Statements: None applicable

  • PPE Requirements:

    • Safety goggles

    • Lab coat

    • Nitrile or latex gloves

  • First Aid Measures:

    • Inhalation: Move to fresh air

    • Skin Contact: Wash with soap and water

    • Eye Contact: Rinse with clean water for several minutes

    • Ingestion: Rinse mouth; seek medical attention if needed

4. Storage & Handling Attributes

  • Storage Conditions:

    • Keep tightly closed in a cool, dry place

    • Avoid exposure to moisture

    • Store away from incompatible substances (e.g., strong acids)

  • Handling Notes:

    • Avoid creating dust

    • Use in well-ventilated areas

    • Wash hands after handling

5. Regulatory & Compliance Attributes

  • EC Number: 231-448-7

  • UN Number: Not regulated

  • Hazard Class: Non-hazardous for transport

  • REACH Status: Registered

6. Laboratory Applications

  • Primary Uses:

    • Buffering agent in chemical and biochemical laboratories

    • Component in phosphate-buffered saline (PBS)

    • Analytical reagent

    • pH regulation in lab experiments

  • Other Uses:

    • Model compound for phosphate equilibrium studies

    • Used in protein precipitation and purification systems
SAFETY HANDLING PRECAUTIONS

SAFETY MEASURES

SAFETY & HANDLING

  • Personal Protective Equipment (PPE):

    • Safety goggles

    • Lab coat

    • Gloves

  • Storage:

    • Store in a cool, dry, well-ventilated area

    • Keep container tightly closed

    • Protect from moisture and incompatible substances (e.g., strong acids)

  • Handling Tips:

    • Minimize dust generation

    • Avoid breathing dust or mist

    • Wash thoroughly after handling

FIRST AID MEASURES

  • Inhalation: Move to fresh air. Seek medical attention if symptoms persist

  • Skin Contact: Wash off with soap and water

  • Eye Contact: Rinse cautiously with water for several minutes

  • Ingestion: Rinse mouth. Do not induce vomiting unless instructed by medical personnel

FIRE & REACTIVITY INFORMATION

  • Flammability: Not flammable

  • Hazardous Decomposition: Sodium oxides, phosphorous oxides

  • Incompatible With: Strong acids, moisture (hygroscopic)