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Field Stain A and B Extra Pure
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SHORT DESCRIPTION
Field Stain A and B Extra Pure are specially formulated biological stains used primarily in hematology and microbiology for rapid differential staining of blood smears and parasites, especially in field conditions. Field Stain A typically contains methylene blue and azure dyes dissolved in a phosphate buffer, which preferentially stains the nuclei and cytoplasm of white blood cells. Field Stain B, on the other hand, contains eosin and counterstains the red blood cells and other acidic cellular components. Together, these stains provide excellent contrast and clarity, making them ideal for identifying malarial parasites and conducting quick diagnostic assessments in low-resource or remote environments. Their extra pure quality ensures minimal contamination, consistent performance, and reliable results in both clinical and educational settings.
Description
USES
Primary Laboratory Uses:
- Microscopic examination of blood smears:
Field Stain A (basic dye – methylene blue/azure) and Field Stain B (acidic dye – eosin) are primarily used together for rapid differential staining of blood films, particularly for identifying malaria parasites, trypanosomes, and other blood-borne pathogens. - Diagnostic hematology:
Vital for differentiating blood cells such as leukocytes, erythrocytes, and platelets under light microscopy in clinical labs and field settings.
Secondary Laboratory Uses:
- Parasitology field testing:
Favored in remote or low-resource settings due to its fast action and minimal need for lab infrastructure, supporting on-site diagnosis of parasitic infections. - Educational and training purposes:
Used in teaching laboratories for demonstrating blood cell morphology and staining techniques to students and trainees in hematology and pathology. - Comparative staining studies:
In research, it is sometimes used for comparing stain quality, efficiency, and morphological clarity with other stains like Giemsa or Wright’s.
KEY PRODUCT FEATURES
KEY ATTRIBUTES
1. Basic Identification Attributes
- Product Name: Field Stain A and Field Stain B
- Type: Biological staining reagents (Romanowsky-type stains)
- Grade: Extra Pure (Laboratory Reagent Grade)
- Appearance:
- Field Stain A: Dark blue to purple solution (methylene blue + Azure I)
- Field Stain B: Reddish solution (Eosin Y in buffer)
2. Composition
- Field Stain A:
- Methylene Blue
- Azure I
- Glycerin
- Solvent (often ethanol or methanol)
- Field Stain B:
- Eosin Y
- Buffered aqueous or alcohol-based solution
3. Physical & Chemical Properties
- Solubility: Fully soluble in alcohols and water (depending on formulation)
- pH Range:
- A: Typically slightly basic
- B: Typically acidic (pH ~5–6)
- Stability: Stable in tightly sealed bottles, light-sensitive
- Storage: Store in cool, dry place away from light
4. Safety & Hazard Attributes
- GHS Classification:
- May contain components harmful if swallowed or inhaled
- Irritant to eyes and skin
- Hazard Statements:
- H315: Causes skin irritation
- H319: Causes serious eye irritation
- H335: May cause respiratory irritation
- PPE Requirements:
- Lab coat
- Nitrile gloves
- Eye protection
- Use under fume hood if aerosolized or heated
- First Aid Measures:
- Inhalation: Fresh air, seek attention if symptoms persist
- Skin Contact: Wash thoroughly with water
- Eye Contact: Flush with water for 15 minutes
- Ingestion: Rinse mouth, do not induce vomiting; seek medical attention
5. Storage & Handling Attributes
- Storage Conditions:
- Keep tightly closed
- Store at room temperature, away from heat and sunlight
- Avoid contamination between bottles
- Handling Notes:
- Shake before use if sedimented
- Use clean applicators to avoid microbial contamination
6. Regulatory & Compliance Attributes
- Field Stain A CAS Numbers (components):
- Methylene Blue: 61-73-4
- Azure I: 531-53-3
- Field Stain B CAS (Eosin Y): 17372-87-1
- Hazard Class: Not regulated as dangerous goods (small quantities)
- UN Number: Not applicable
7. Laboratory Applications
- Primary Uses:
- Hematological staining of blood smears, especially for malaria parasite detection
- Commonly used in field microscopy
- Differential staining of cytoplasm and nuclei
- Secondary Uses:
- Quick diagnostics in medical field labs
- Educational demonstrations of blood smear techniques
- Detection of protozoa and cell morphology in biological samples
SAFETY HANDLING PRECAUTIONS
SAFETY MEASURES
SAFETY & HANDLING
- May cause skin, eye, or respiratory irritation
- Use gloves and eye protection when handling
- Work in a well-ventilated area
Storage:
- Keep in tightly sealed amber bottles
- Store at room temperature, away from direct light and heat
FIRST AID MEASURES
- Inhalation: Move to fresh air
- Eye Contact: Rinse cautiously with water for several minutes
- Skin Contact: Wash off with soap and water
- Ingestion: Rinse mouth, seek medical attention if symptoms persist
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