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Phenolphthalein Solution 500ml

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Phenolphthalein is a chemical compound commonly used as a pH indicator in titrations and other analytical chemistry applications. It is a colorless crystalline solid that is often dissolved in alcohol to create a phenolphthalein solution.

Properties of Phenolphthalein Solution

  1. pH Indicator:
    • Acidic Solutions (pH < 8.2): Colorless
    • Slightly Basic Solutions (pH 8.2 – 10.0): Pink to fuchsia
    • Strongly Basic Solutions (pH > 10.0): Colorless
  2. Solubility: Phenolphthalein is soluble in alcohol and slightly soluble in water.

Preparation of Phenolphthalein Solution

  1. Ingredients:
    • Phenolphthalein powder
    • Ethanol (or another suitable alcohol)
    • Distilled water
  2. Procedure:
    • Dissolve 1 gram of phenolphthalein powder in 100 mL of ethanol.
    • Dilute the solution with distilled water to make a 1% phenolphthalein solution (typically 100 mL of the ethanol solution to 900 mL of water).

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Phenolphthalein Solution

Phenolphthalein is widely used in laboratories, particularly in the fields of chemistry and biology, due to its reliable and distinct color change properties. Here are some of its key laboratory uses:

1. Acid-Base Titrations

Phenolphthalein is commonly used as an indicator in acid-base titrations due to its clear and distinct color change:

  • Procedure: A known concentration of an acid or base is slowly added to a solution containing phenolphthalein until a color change indicates that neutralization has occurred.
  • Endpoint Detection: The solution changes from colorless to pink as the pH moves from acidic to slightly basic (around pH 8.2-10.0). Beyond pH 10.0, the solution becomes colorless again.

2. pH Indicator

Phenolphthalein is used to determine the pH range of a solution:

  • Colorless in acidic solutions (pH < 8.2)
  • Pink in slightly basic solutions (pH 8.2-10.0)
  • Colorless in strongly basic solutions (pH > 10.0)

3. Qualitative Analysis

In analytical chemistry, phenolphthalein can be used for qualitative analysis:

  • Detecting Carbonate Ions: Adding phenolphthalein to a solution containing carbonate ions will result in a pink color if the solution is basic.
  • Alkalinity Test: Used to test for the presence of basic compounds in various solutions.

4. Visualizing Chemical Reactions

Phenolphthalein is used to visualize certain chemical reactions, particularly those involving neutralization and changes in pH:

  • Demonstrating Neutralization: Used in educational settings to visually demonstrate the neutralization of acids and bases.

5. Medical and Biological Uses

Though not as common today, phenolphthalein has been used in biological research and diagnostics:

  • Laxatives: Historically used in laxatives, though now less common due to safety concerns.
  • Cell Viability Assays: Sometimes used in biological assays to assess cell viability.

6. Leachate Testing

Phenolphthalein is used in environmental science to test for leachates:

  • Concrete Testing: Used to detect the presence of calcium hydroxide in concrete leachates. The solution turns pink in the presence of hydroxide ions, indicating basic conditions.

7. Amateur and Educational Chemistry

Phenolphthalein is popular in educational laboratories for teaching purposes:

  • Chemistry Demonstrations: Used in various experiments to demonstrate chemical principles such as acid-base reactions, pH changes, and titration techniques.

 

SKU: ACS82792CHEM0 Category:

When handling phenolphthalein in a laboratory setting, it is important to adhere to safety precautions to minimize exposure and prevent accidents. Here are detailed safety guidelines:

Personal Protective Equipment (PPE)

  1. Gloves: Wear appropriate chemical-resistant gloves (e.g., nitrile gloves) to prevent skin contact.
  2. Safety Goggles: Use safety goggles or a face shield to protect your eyes from splashes.
  3. Lab Coat: Wear a lab coat or apron to protect your skin and clothing.
  4. Closed-toe Shoes: Ensure your feet are protected by wearing closed-toe shoes.

Handling and Storage

  1. Ventilation: Work in a well-ventilated area, preferably under a fume hood, to avoid inhaling fumes, especially if working with the alcoholic solution.
  2. Avoid Ingestion and Inhalation: Do not eat, drink, or smoke in areas where phenolphthalein is used or stored. Avoid inhaling dust or fumes.
  3. Storage: Store phenolphthalein in a tightly closed container, away from incompatible substances like strong acids and bases. Keep it in a cool, dry, and well-ventilated area away from direct sunlight and heat sources.
  4. Labeling: Clearly label all containers holding phenolphthalein solutions with appropriate hazard symbols and safety information.

First Aid Measures

  1. Skin Contact: In case of skin contact, immediately wash with plenty of soap and water. Remove contaminated clothing and seek medical attention if irritation persists.
  2. Eye Contact: If phenolphthalein gets into the eyes, rinse immediately with plenty of water for at least 15 minutes and seek medical attention.
  3. Inhalation: If inhaled, move the person to fresh air. If breathing is difficult, give oxygen and seek medical attention.
  4. Ingestion: If ingested, do not induce vomiting. Rinse the mouth with water and seek immediate medical attention.

Spill and Accident Procedures

  1. Small Spills: Absorb small spills with inert material (e.g., sand, vermiculite) and place in a chemical waste container. Clean the area with water and detergent.
  2. Large Spills: Evacuate the area and contain the spill. Use appropriate personal protective equipment and absorb with inert material. Dispose of the material according to local regulations.
  3. Emergency Equipment: Ensure that emergency showers, eyewash stations, and fire extinguishers are accessible in the area where phenolphthalein is used.

Waste Disposal

  1. Hazardous Waste: Dispose of phenolphthalein and its solutions as hazardous waste according to local, state, and federal regulations.
  2. Container Disposal: Empty containers should be rinsed and disposed of properly, following hazardous waste protocols.

Environmental Precautions

  1. Prevent Release: Do not release phenolphthalein into the environment. Prevent spills from entering drains or water sources.
  2. Proper Disposal: Ensure that waste is properly labeled and disposed of at authorized disposal facilities.

 

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