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Brilliant Blue Extra Pure

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Brilliant Blue Extra Pure is a high-purity synthetic dye extensively used in laboratory research, particularly in biochemistry, molecular biology, and microbiology. It is commonly employed as a protein stain in techniques such as SDS-PAGE, where it binds to proteins for visualization and quantification. Additionally, it is used in spectrophotometric assays, as a pH indicator, and in dye tracing studies to monitor fluid movement or diffusion. The extra pure grade ensures consistent dye intensity, minimal impurities, and high solubility, making it ideal for precise analytical and experimental applications. It should be stored in a cool, dry environment, protected from light and tightly sealed to maintain its color integrity and stability.

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Categories: Analytical Reagents, Biochemical Reagents Tags: BiochemicalReagent, BradfordAssay, BrilliantBlue, CellStaining, HighPurityDye, LabGradeColorant, MolecularBiologyDye, ProteinStaining

Description

Table of Contents

Brilliant Blue Extra Pure

Primary Uses

Protein Staining in Gel Electrophoresis
- Widely used as a biological stain in techniques like SDS-PAGE for visualizing proteins, especially in the form of Coomassie Brilliant Blue G-250 or R-250.
pH Indicator or Tracking Dye
- Acts as a tracking dye in electrophoresis buffer systems to monitor the progress of DNA or protein migration.
Spectrophotometric Analysis
- Used in colorimetric assays for quantitative protein determination, such as the Bradford assay, due to its binding and absorption shift upon protein interaction.

Secondary Uses

Model Compound for Dye Adsorption Studies
- Employed in environmental chemistry labs to study adsorption, desorption, and filtration behavior of dyes on various media like activated carbon or clay.
Demonstrations in Analytical Chemistry
- Used in chromatography demonstrations, including paper chromatography, to illustrate migration and separation principles of polar/non-polar compounds.
Staining Agent in Microscopy
- Occasionally used for cytoplasmic or general tissue staining, especially in teaching labs to demonstrate cell structure.

Additional information

PACK SIZE	25 grams Plastic Tin

KEY PRODUCT FEATURES

1. Basic Identification Attributes

Product Name: Brilliant Blue Extra Pure
Other Names: Brilliant Blue FCF, FD&C Blue No. 1, Acid Blue 9
Chemical Formula: C₃₇H₃₄N₂Na₂O₉S₃
CAS Number: 3844-45-9
HS Code: 3204.12.94
Grade: Extra Pure (suitable for laboratory and analytical applications)

2. Physical & Chemical Properties

Physical State: Powder or granules
Color & Odor: Deep blue; odorless
Molecular Weight: ~792.84 g/mol (sodium salt)
Melting Point: Decomposes
Solubility in Water: Highly soluble (forms a blue solution)
Solubility in Alcohol: Poorly soluble
pH Range (1% solution): 5.0–7.0
Light Stability: Fair to good (light-sensitive under extreme exposure)
Staining Ability: Strong dye; stains surfaces and skin easily
UV Absorption: Max absorbance at ~630 nm

3. Safety & Hazard Attributes

GHS Classification:
- Not classified as hazardous under GHS (for lab-scale use)
GHS Pictograms: None required
NFPA Ratings:
- Health: 1
- Flammability: 0
- Reactivity: 0
PPE Requirements:
- Lab coat, gloves, safety goggles
Inhalation/Ingestion Risks:
- Low acute toxicity; avoid inhalation of dust
Skin/Eye Contact:
- May cause mild irritation; use proper protection

4. Storage & Handling Attributes

Storage Conditions:
- Store in tightly sealed containers
- Keep in a cool, dry, well-ventilated place
Handling Precautions:
- Avoid dust generation and contact with eyes
- Clean spills promptly to prevent staining
Shelf Life:
- Stable under recommended storage for several years

5. Regulatory & Compliance Attributes

Regulatory Status:
- Approved as a food dye (FD&C Blue No. 1), but lab grade not intended for consumption
- Not classified as hazardous under transport regulations
UN Number: Not regulated
Disposal Guidelines:
- Dispose in accordance with local environmental regulations
- May be flushed with excess water if permitted

6. Environmental & Health Impact

Ecotoxicity:
- May be harmful in large amounts to aquatic life
Biodegradability:
- Not readily biodegradable
Carcinogenicity/Mutagenicity:
- No known carcinogenic effects in lab usage context
Health Concerns:
- Minimal for standard lab exposure; not for ingestion or injection

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):
- Wear lab coat, chemical-resistant gloves, and safety goggles.
- If working with powder, wear a dust mask or respirator to avoid inhalation.
Handling:
- Handle in a well-ventilated lab or under a fume hood.
- Avoid contact with skin, eyes, and clothing.
- Minimize dust generation and accumulation.
Storage:
- Store in a tightly sealed container.
- Keep in a cool, dry place away from light and incompatible substances (strong oxidizers).
Incompatibilities:
- Avoid contact with strong oxidizing agents and strong acids.

FIRST AID MEASURES

Inhalation:
- Move the person to fresh air.
- If breathing is difficult, administer oxygen and get medical attention.
Skin Contact:
- Wash with plenty of soap and water.
- Remove contaminated clothing and wash before reuse.
Eye Contact:
- Rinse cautiously with water for at least 15 minutes.
- Seek medical attention if irritation or symptoms persist.
Ingestion:
- Rinse mouth thoroughly with water.
- Do not induce vomiting. Seek medical help, especially if large quantities are swallowed.

FIRE FIGHTING MEASURES

Extinguishing Media:
- Use water spray, dry chemical, foam, or CO₂.
Fire Hazards:
- Not highly flammable but may decompose under fire conditions, producing toxic fumes (e.g., nitrogen oxides, carbon monoxide).
Firefighter Protection:
- Use self-contained breathing apparatus (SCBA) and full protective gear.