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Brilliant Blue Extra Pure
Original price was: $ 18.00.$ 17.21Current price is: $ 17.21.
Brilliant Blue Extra Pure is a high-purity synthetic dye extensively used in laboratory research, particularly in biochemistry, molecular biology, and microbiology. It is commonly employed as a protein stain in techniques such as SDS-PAGE, where it binds to proteins for visualization and quantification. Additionally, it is used in spectrophotometric assays, as a pH indicator, and in dye tracing studies to monitor fluid movement or diffusion. The extra pure grade ensures consistent dye intensity, minimal impurities, and high solubility, making it ideal for precise analytical and experimental applications. It should be stored in a cool, dry environment, protected from light and tightly sealed to maintain its color integrity and stability.
Brilliant Blue Extra Pure
Primary Uses
- Protein Staining in Gel Electrophoresis
- Widely used as a biological stain in techniques like SDS-PAGE for visualizing proteins, especially in the form of Coomassie Brilliant Blue G-250 or R-250.
- pH Indicator or Tracking Dye
- Acts as a tracking dye in electrophoresis buffer systems to monitor the progress of DNA or protein migration.
- Spectrophotometric Analysis
- Used in colorimetric assays for quantitative protein determination, such as the Bradford assay, due to its binding and absorption shift upon protein interaction.
Secondary Uses
- Model Compound for Dye Adsorption Studies
- Employed in environmental chemistry labs to study adsorption, desorption, and filtration behavior of dyes on various media like activated carbon or clay.
- Demonstrations in Analytical Chemistry
- Used in chromatography demonstrations, including paper chromatography, to illustrate migration and separation principles of polar/non-polar compounds.
- Staining Agent in Microscopy
- Occasionally used for cytoplasmic or general tissue staining, especially in teaching labs to demonstrate cell structure.
| PACK SIZE | 25 grams Plastic Tin |
|---|
1. Basic Identification Attributes
- Product Name: Brilliant Blue Extra Pure
- Other Names: Brilliant Blue FCF, FD&C Blue No. 1, Acid Blue 9
- Chemical Formula: C₃₇H₃₄N₂Na₂O₉S₃
- CAS Number: 3844-45-9
- HS Code: 3204.12.94
- Grade: Extra Pure (suitable for laboratory and analytical applications)
2. Physical & Chemical Properties
- Physical State: Powder or granules
- Color & Odor: Deep blue; odorless
- Molecular Weight: ~792.84 g/mol (sodium salt)
- Melting Point: Decomposes
- Solubility in Water: Highly soluble (forms a blue solution)
- Solubility in Alcohol: Poorly soluble
- pH Range (1% solution): 5.0–7.0
- Light Stability: Fair to good (light-sensitive under extreme exposure)
- Staining Ability: Strong dye; stains surfaces and skin easily
- UV Absorption: Max absorbance at ~630 nm
3. Safety & Hazard Attributes
- GHS Classification:
- Not classified as hazardous under GHS (for lab-scale use)
- GHS Pictograms: None required
- NFPA Ratings:
- Health: 1
- Flammability: 0
- Reactivity: 0
- PPE Requirements:
- Lab coat, gloves, safety goggles
- Inhalation/Ingestion Risks:
- Low acute toxicity; avoid inhalation of dust
- Skin/Eye Contact:
- May cause mild irritation; use proper protection
4. Storage & Handling Attributes
- Storage Conditions:
- Store in tightly sealed containers
- Keep in a cool, dry, well-ventilated place
- Handling Precautions:
- Avoid dust generation and contact with eyes
- Clean spills promptly to prevent staining
- Shelf Life:
- Stable under recommended storage for several years
5. Regulatory & Compliance Attributes
- Regulatory Status:
- Approved as a food dye (FD&C Blue No. 1), but lab grade not intended for consumption
- Not classified as hazardous under transport regulations
- UN Number: Not regulated
- Disposal Guidelines:
- Dispose in accordance with local environmental regulations
- May be flushed with excess water if permitted
6. Environmental & Health Impact
- Ecotoxicity:
- May be harmful in large amounts to aquatic life
- Biodegradability:
- Not readily biodegradable
- Carcinogenicity/Mutagenicity:
- No known carcinogenic effects in lab usage context
- Health Concerns:
- Minimal for standard lab exposure; not for ingestion or injection
SAFETY PRECAUTIONS
- Personal Protective Equipment (PPE):
- Wear lab coat, chemical-resistant gloves, and safety goggles.
- If working with powder, wear a dust mask or respirator to avoid inhalation.
- Handling:
- Handle in a well-ventilated lab or under a fume hood.
- Avoid contact with skin, eyes, and clothing.
- Minimize dust generation and accumulation.
- Storage:
- Store in a tightly sealed container.
- Keep in a cool, dry place away from light and incompatible substances (strong oxidizers).
- Incompatibilities:
- Avoid contact with strong oxidizing agents and strong acids.
FIRST AID MEASURES
- Inhalation:
- Move the person to fresh air.
- If breathing is difficult, administer oxygen and get medical attention.
- Skin Contact:
- Wash with plenty of soap and water.
- Remove contaminated clothing and wash before reuse.
- Eye Contact:
- Rinse cautiously with water for at least 15 minutes.
- Seek medical attention if irritation or symptoms persist.
- Ingestion:
- Rinse mouth thoroughly with water.
- Do not induce vomiting. Seek medical help, especially if large quantities are swallowed.
FIRE FIGHTING MEASURES
- Extinguishing Media:
- Use water spray, dry chemical, foam, or CO₂.
- Fire Hazards:
- Not highly flammable but may decompose under fire conditions, producing toxic fumes (e.g., nitrogen oxides, carbon monoxide).
- Firefighter Protection:
- Use self-contained breathing apparatus (SCBA) and full protective gear.
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