

Immersion Oil 50ml
$3,500.00 Original price was: $3,500.00.$3,000.00Current price is: $3,000.00.
Immersion oil is a specialized oil used in microscopy to enhance the clarity and resolution of images observed through high-power objective lenses. It has a refractive index similar to that of glass, reducing light refraction and increasing the amount of light reaching the microscope’s objective lens. This allows for greater detail and higher magnification of microscopic specimens. Typically, immersion oil is used with 100x oil-immersion objectives, and it’s essential for achieving the highest possible image quality in certain types of microscopy.
Uses of Immersion Oil
Microscopy:
To improve the clarity and resolution of images when using high magnification objectives (typically 100x). It helps to minimize light refraction and improve image sharpness.
Cellular and Microbial Studies:
To observe fine details of cells, microorganisms, and tissues that are not visible with lower magnification objectives.
Specimen Examination:
To facilitate the examination of delicate or transparent specimens by providing a continuous optical path between the specimen and the lens.
Enhanced Contrast:
To increase the contrast of images, making it easier to distinguish between different structures within the specimen.
High-Resolution Imaging:
To support advanced imaging techniques that require the highest possible resolution and detail.
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Properties
- Color: Red to purplish-red.
- Solubility: Soluble in water and ethanol.
- Staining Characteristics: Stains chromatin and cytoplasmic components, providing contrast for better visualization under a microscope.
Preparation
- Ingredients:
- Carmine dye: A natural red dye extracted from the cochineal insect.
- Acetic acid: A colorless liquid organic compound with a pungent smell.
- Procedure:
- Dissolve a specific amount of carmine powder in hot distilled water.
- Add glacial acetic acid to the solution.
- Filter the mixture to remove any undissolved particles.
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Properties
- Chemical Formula: NH4C2H3O2
- Molecular Weight: 77.08 g/mol
- Appearance: White, crystalline solid
- Solubility: Highly soluble in water
- Melting Point: Decomposes upon heating
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