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Immersion Oil
Immersion Oil is a high-purity, optically transparent oil used primarily in microscopy to enhance the resolution of high-power objectives. Applied between the microscope objective lens and the cover glass, immersion oil reduces light refraction and increases numerical aperture, allowing for clearer and more detailed imaging. It is colorless, non-drying, and has a refractive index closely matching that of glass (typically around 1.515).
This 50ml volume is ideal for laboratory, clinical, and educational settings where precision microscopy work is performed. Immersion oil is formulated to be chemically stable, non-drying, and safe for delicate optics when used correctly.
Immersion Oil
Primary Uses
- Laboratory and Scientific Applications
- Used with high-magnification (usually 100x or higher) oil-immersion microscope objectives.
- Reduces light refraction between slide and objective lens for enhanced image clarity.
- Essential in biological, medical, and pathology labs for detailed cell and tissue visualization.
- Applied in educational institutions for advanced biological microscopy demonstrations.
- Common in industrial inspection of fine materials, electronics, or metallurgical specimens.
Secondary Uses
- Optical Equipment Maintenance
- Occasionally used in calibration or testing of optical systems where refractive index matching is required (under expert supervision).
1. Basic Identification Attributes
- Common/Trade Name: Immersion Oil
- CAS Number: 8042-47-5 (for mineral-oil-based formulations)
- Refractive Index: ~1.515 at 23°C
- HS Code: 3824.99
- Synonyms: Microscopy Oil, Oil for Immersion Objectives
2. Physical & Chemical Properties
- Physical State: Clear, colorless liquid
- Odor: Odorless or very mild hydrocarbon scent
- Viscosity: Medium to high, depending on formulation
- Solubility: Insoluble in water
- Compatibility: Glass and most optical lenses (verify lens coating safety)
- Volatility: Low; non-drying
3. Safety & Hazard Attributes
- GHS Classification: Not classified as hazardous under normal use
- Toxicity: Low; not intended for ingestion or inhalation
- Allergen Information: No known allergens in standard formulations
- Flash Point: > 150°C (varies by formulation)
4. Storage & Handling Attributes
- Storage Conditions: Store tightly sealed in a cool, dry, dust-free place
- Container Type: Amber glass or opaque plastic to avoid UV degradation
- Shelf Life: 3–5 years under ideal storage conditions
- Handling Precautions: Avoid contamination and exposure to air when not in use
5. Regulatory & Compliance Attributes
- Complies with ISO standards for immersion microscopy
- Produced in ISO or GMP-certified facilities
- Non-reactive and safe for use with most optical instruments
- Not intended for food, pharmaceutical, or cosmetic use
6. Environmental & Health Impact
- Biodegradability: Partially biodegradable depending on formulation
- Ecotoxicity: Low when used and disposed of properly
- Bioaccumulation: Not expected in normal use scenarios
Safety Handling Precautions
- PPE Required: Gloves and lab coat recommended during prolonged handling
- Handling Guidelines: Use dropper or applicator; avoid spills on microscope parts
First Aid Measures
- Inhalation: Move to fresh air; unlikely to pose risk
- Skin Contact: Wash with soap and water if contact occurs
- Eye Contact: Rinse gently with clean water
- Ingestion: Seek medical attention; not meant for internal use
Firefighting Measures
- Fire Hazards: Slightly flammable at high temperatures
- Extinguishing Media: CO₂, dry chemical, or foam
- Special Precautions: Avoid using water jet directly on burning oil
- Hazardous Combustion Products: Carbon monoxide, carbon dioxide
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