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Immersion Oil

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Immersion Oil is a high-purity, optically transparent oil used primarily in microscopy to enhance the resolution of high-power objectives. Applied between the microscope objective lens and the cover glass, immersion oil reduces light refraction and increases numerical aperture, allowing for clearer and more detailed imaging. It is colorless, non-drying, and has a refractive index closely matching that of glass (typically around 1.515).

This 50ml volume is ideal for laboratory, clinical, and educational settings where precision microscopy work is performed. Immersion oil is formulated to be chemically stable, non-drying, and safe for delicate optics when used correctly.

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Categories: SPECIALTY AND FINE CHEMICALS, Specialty Laboratory Chemicals(Special Laboratory Equipment) Tags: High Refractive Index Oil, Immersion Oil, Laboratory Imaging Reagent, Microscope Lens Oil, Microscopy Oil, Optical Resolution Enhancer

Description

Table of Contents

Immersion Oil

Primary Uses

Laboratory and Scientific Applications

Used with high-magnification (usually 100x or higher) oil-immersion microscope objectives.
Reduces light refraction between slide and objective lens for enhanced image clarity.
Essential in biological, medical, and pathology labs for detailed cell and tissue visualization.
Applied in educational institutions for advanced biological microscopy demonstrations.
Common in industrial inspection of fine materials, electronics, or metallurgical specimens.

Secondary Uses

Optical Equipment Maintenance

Occasionally used in calibration or testing of optical systems where refractive index matching is required (under expert supervision).

KEY PRODUCT FEATURES

1. Basic Identification Attributes

Common/Trade Name: Immersion Oil
CAS Number: 8042-47-5 (for mineral-oil-based formulations)
Refractive Index: ~1.515 at 23°C
HS Code: 3824.99
Synonyms: Microscopy Oil, Oil for Immersion Objectives

2. Physical & Chemical Properties

Physical State: Clear, colorless liquid
Odor: Odorless or very mild hydrocarbon scent
Viscosity: Medium to high, depending on formulation
Solubility: Insoluble in water
Compatibility: Glass and most optical lenses (verify lens coating safety)
Volatility: Low; non-drying

3. Safety & Hazard Attributes

GHS Classification: Not classified as hazardous under normal use
Toxicity: Low; not intended for ingestion or inhalation
Allergen Information: No known allergens in standard formulations
Flash Point: > 150°C (varies by formulation)

4. Storage & Handling Attributes

Storage Conditions: Store tightly sealed in a cool, dry, dust-free place
Container Type: Amber glass or opaque plastic to avoid UV degradation
Shelf Life: 3–5 years under ideal storage conditions
Handling Precautions: Avoid contamination and exposure to air when not in use

5. Regulatory & Compliance Attributes

Complies with ISO standards for immersion microscopy
Produced in ISO or GMP-certified facilities
Non-reactive and safe for use with most optical instruments
Not intended for food, pharmaceutical, or cosmetic use

6. Environmental & Health Impact

Biodegradability: Partially biodegradable depending on formulation
Ecotoxicity: Low when used and disposed of properly
Bioaccumulation: Not expected in normal use scenarios

SAFETY HANDLING PRECAUTIONS

Safety Handling Precautions

PPE Required: Gloves and lab coat recommended during prolonged handling
Handling Guidelines: Use dropper or applicator; avoid spills on microscope parts