PAPER CHROMATOGRAPHY
Product Description
Paper Chromatography is an analytical laboratory technique used to separate and identify mixtures of substances based on their differential migration across a specially treated paper medium. It is widely used for qualitative analysis of compounds such as pigments, amino acids, and pharmaceuticals. The process involves applying a small sample spot onto chromatography paper and allowing a solvent to travel through the paper by capillary action, separating the components according to their solubility and affinity for the paper. This method is simple, cost-effective, and valuable in biochemical, pharmaceutical, and environmental laboratories for analyzing complex mixtures.
Primary Uses
Laboratory and Analytical Applications
- Separating and identifying components in chemical mixtures.
- Analyzing plant pigments, dyes, and inks.
- Qualitative testing in pharmaceutical and biochemical research.
- Monitoring purity of substances and reaction progress.
Secondary Uses
Educational and Research Purposes
- Demonstrating chromatographic principles in teaching laboratories.
- Preliminary analysis before more advanced chromatographic techniques.
Product Description
Paper Chromatography is an analytical laboratory technique used to separate and identify mixtures of substances based on their differential migration across a specially treated paper medium. It is widely used for qualitative analysis of compounds such as pigments, amino acids, and pharmaceuticals. The process involves applying a small sample spot onto chromatography paper and allowing a solvent to travel through the paper by capillary action, separating the components according to their solubility and affinity for the paper. This method is simple, cost-effective, and valuable in biochemical, pharmaceutical, and environmental laboratories for analyzing complex mixtures.
Uses
Primary Uses
Laboratory and Analytical Applications
- Separating and identifying components in chemical mixtures.
- Analyzing plant pigments, dyes, and inks.
- Qualitative testing in pharmaceutical and biochemical research.
- Monitoring purity of substances and reaction progress.
Secondary Uses
Educational and Research Purposes
- Demonstrating chromatographic principles in teaching laboratories.
- Preliminary analysis before more advanced chromatographic techniques.
Key Attributes
Basic Identification Attributes
- Medium: Special chromatography filter paper.
- Solvent: Various solvents depending on sample type.
- Process: Capillary action-driven separation.
- Detection: Visual, UV light, or chemical reagents for spot visualization.
Physical & Chemical Properties
- Paper: High purity, uniform thickness, and absorbency.
- Solvent Compatibility: Compatible with a wide range of solvents.
Safety & Hazard Attributes
- Handle solvents and chemicals with care due to flammability and toxicity risks.
- Use in well-ventilated areas.
Storage & Handling Attributes
- Store chromatography paper in a dry, dust-free environment.
- Protect solvents from evaporation and contamination.
Regulatory & Compliance Attributes
- Manufactured to meet laboratory analytical standards.
Environmental & Health Impact
- Solvent disposal must comply with hazardous waste regulations.
- Paper is biodegradable; proper disposal recommended.
Safety Handling Precautions
- Use gloves and eye protection when handling solvents.
- Avoid inhalation of solvent vapors.
First Aid Measures
- In case of solvent exposure, move to fresh air and wash affected skin.
- Seek medical attention if irritation or poisoning occurs.
Firefighting Measures
- Solvents used may be flammable; use appropriate extinguishers (foam, CO₂).
- Keep away from ignition sources.