

Sucrose 500g
$360.00 Original price was: $360.00.$250.00Current price is: $250.00.
Sucrose is a type of sugar commonly found in many plants, including sugarcane and sugar beets. It’s a disaccharide, meaning it’s composed of two simpler sugars, glucose and fructose, linked together. Sucrose is often used as a sweetener in food and drinks, and it’s what we typically refer to as “table sugar.” When we consume sucrose, enzymes in our digestive system break it down into glucose and fructose, which our bodies can then use for energy.
Uses of Sucrose
Culture Media:
Sucrose is used in microbiology laboratories to prepare culture media for growing microorganisms. It provides a carbon source for microbial growth.
Density Gradient Centrifugation:
Sucrose solutions are commonly used in density gradient centrifugation to separate particles based on their density. Different concentrations of sucrose solutions create layers of varying densities, allowing for the separation of particles or molecules based on their buoyancy.
Cryopreservation:
Sucrose can be utilized in cryopreservation solutions to protect biological samples, such as cells or tissues, during freezing and storage. It helps prevent the formation of ice crystals, which can damage cellular structures.
Protein Purification:
Sucrose density gradients are often employed in protein purification techniques, such as density gradient ultracentrifugation, to separate and purify proteins based on their molecular weights or densities.
Osmolarity Control:
Sucrose solutions are used in laboratory experiments to control osmotic pressure. By adjusting the concentration of sucrose in a solution, researchers can create environments with specific osmotic properties for various biological or chemical studies.
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Properties
- Color: Red to purplish-red.
- Solubility: Soluble in water and ethanol.
- Staining Characteristics: Stains chromatin and cytoplasmic components, providing contrast for better visualization under a microscope.
Preparation
- Ingredients:
- Carmine dye: A natural red dye extracted from the cochineal insect.
- Acetic acid: A colorless liquid organic compound with a pungent smell.
- Procedure:
- Dissolve a specific amount of carmine powder in hot distilled water.
- Add glacial acetic acid to the solution.
- Filter the mixture to remove any undissolved particles.
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Properties
- Chemical Formula: NH4C2H3O2
- Molecular Weight: 77.08 g/mol
- Appearance: White, crystalline solid
- Solubility: Highly soluble in water
- Melting Point: Decomposes upon heating
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