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Bromothymol Blue Extra Pure

Original price was: $ 18.00.Current price is: $ 17.00.

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Bromothymol Blue Extra Pure is a high-purity pH indicator dye widely used in laboratory experiments, especially in acid-base titrations and biological research. It exhibits a distinct color change from yellow in acidic solutions (pH < 6.0) to blue in basic conditions (pH > 7.6), making it valuable for monitoring pH changes in aqueous systems. This dye is also employed in respiratory experiments to detect CO₂ levels through pH shifts. The extra pure grade ensures excellent color stability, high sensitivity, and minimal impurities, making it suitable for precise analytical and educational applications. It should be stored in a tightly closed container, protected from light and moisture to maintain its integrity.

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Categories: Analytical Reagents, Biochemical Reagents Tags: AcidBaseIndicator, AnalyticalReagent, BiochemicalStudies, BromothymolBlue, BufferSolutions, LabGradeIndicator, PHIndicator, SpectroscopyDye

Description

Table of Contents

Bromothymol Blue Extra Pure

Primary Uses

pH Indicator in Acid–Base Titrations
- Changes from yellow (pH < 6.0) to blue (pH > 7.6)—ideal for titrations near neutral pH (e.g., weak acid vs. strong base).
Buffer System Studies
- Used in buffer capacity experiments and to visually monitor pH stability in buffer solutions during kinetic or enzymatic studies.
Respiration and Photosynthesis Experiments
- Applied in biology labs to detect CO₂ levels:
  - Yellow in acidic solutions (CO₂ dissolved)
  - Blue in basic or neutral solutions
- Helps monitor cellular respiration or photosynthetic CO₂ uptake.
Colorimetric Assays and Visual pH Checks
- Incorporated into colorimetric kits for rapid visual estimation of pH, especially in soil, water, and biochemical media testing.

Secondary Uses

Demonstration of pH Concept in Teaching Labs
- Great for classroom demonstrations showing pH shifts and acid-base neutralization visually.
Biological Culture Monitoring
- Sometimes added to cell culture media or fermentation broths to visually signal pH changes due to microbial metabolism.
Spectrophotometric Calibration
- Used in UV-Vis experiments for studying absorbance spectra of indicators, or calibrating spectrophotometers.

Additional information

PACK SIZE	500 grams Plastic Tin

KEY PRODUCT FEATURES

1. Basic Identification Attributes

Chemical Name: Bromothymol Blue
CAS Number: 76-59-5
HS Code: 2934.99.90
Molecular Formula: C₂₇H₂₈Br₂O₅S
Molecular Weight: 624.38 g/mol
Synonyms: BTB, Bromothymol sulfone phthalein
Grade: Extra Pure (for analytical/laboratory reagent use)

2. Physical & Chemical Properties

Appearance: Dark green to bluish powder or crystals
Odor: Odorless
Solubility: Slightly soluble in water; soluble in ethanol and dilute alkali solutions
pH Range (Color Change):
- Yellow below pH 6.0
- Green at pH ~7.0
- Blue above pH 7.6
Melting Point: Decomposes above 200 °C (no clear melting point)
Stability: Stable under normal lab conditions when kept dry and sealed

3. Safety & Hazard Attributes

GHS Classification:
- Not classified as hazardous under GHS in low quantities
GHS Pictograms: None required for typical lab-scale use
NFPA Ratings:
- Health: 1
- Flammability: 0
- Reactivity: 0
Health Hazards:
- May cause mild irritation to eyes, skin, or respiratory tract
Protective Measures:
- Use lab gloves, goggles, and standard ventilation

4. Storage & Handling Attributes

Storage Conditions:
- Store in a tightly sealed container, in a cool, dry, dark place
- Protect from moisture and strong oxidizers
Incompatible Materials:
- Strong acids, oxidizers
Packaging: Amber glass or HDPE bottles with desiccants
Handling Precautions:
- Avoid dust formation; handle with care to maintain reagent purity

5. Regulatory & Compliance Attributes

Regulatory Status:
- Listed under TSCA and other chemical inventories
Transport Classification:
- Not regulated for transport under standard laboratory quantities
Waste Disposal:
- Small quantities may be disposed of in regular lab chemical waste
- Follow local institutional chemical disposal guideline

6. Environmental & Health Impact

Ecotoxicity:
- Low ecological risk at laboratory scale
Biodegradability:
- Slowly degrades in the environment
Bioaccumulation:
- Not expected to bioaccumulate
Health Effects:
- Minimal unless ingested or in case of prolonged exposure

SAFETY HANDLING PRECAUTIONS

SAFETY PRECAUTIONS

Personal Protective Equipment (PPE):
- Wear safety goggles, lab gloves (nitrile recommended), and a lab coat.
- Handle powder in a fume hood to avoid inhalation.
Handling:
- Avoid generation of dust.
- Prevent contact with skin, eyes, and clothing.
- Wash hands thoroughly after use.
Storage:
- Store in a cool, dry, and well-ventilated area.
- Keep the container tightly closed and protected from light.
- Keep away from incompatible materials such as strong oxidizing agents.
Incompatibilities:
- Avoid mixing with strong acids or oxidizers.

FIRST AID MEASURES

Inhalation:
- Remove to fresh air.
- Seek medical attention if symptoms such as coughing or shortness of breath occur.
Skin Contact:
- Wash immediately with plenty of water and soap.
- Remove contaminated clothing.
- Seek medical advice if irritation persists.
Eye Contact:
- Rinse thoroughly with plenty of water for 15 minutes, lifting eyelids.
- Seek medical attention if irritation or vision issues persist.
Ingestion:
- Rinse mouth with water.
- Do not induce vomiting.
- Get medical attention, especially if large amounts were swallowed.

FIRE FIGHTING MEASURES

Flammability:
- Not classified as flammable, but may burn if exposed to fire.
Extinguishing Media:
- Use water spray, foam, carbon dioxide, or dry chemical.
Hazardous Combustion Products:
- May release carbon oxides (CO, CO₂) and sulfur oxides under fire conditions.
Firefighting Equipment:
- Use self-contained breathing apparatus (SCBA) and protective gear.
- Cool containers with water if exposed to fire.