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Ferric Sulphate 500gm
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Formaldehyde 2.5 litres (formalin)
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Field Stain A and B 25gm
Original price was: KSh1,600.00.KSh1,500.00Current price is: KSh1,500.00.
Field Stain A and B are used in a staining technique for blood films, particularly for the rapid identification of malaria parasites and other hematological purposes. The staining method is known as Field’s stain, and it is a type of Romanowsky stain.
Field Stain A
- Composition: Field Stain A is typically an aqueous solution of methylene blue, which stains the nuclei of cells blue.
- Function: It primarily stains the acidic components of the cell, including DNA and RNA. This makes the nuclei and certain cytoplasmic components appear blue.
Field Stain B
- Composition: Field Stain B is usually an aqueous solution of eosin, which stains the basic components of the cell pink.
- Function: It stains the cytoplasm and extracellular components pink to red. Eosinophilic structures, such as red blood cells and certain granules, are highlighted by this stain.
Usage
- Procedure:
- Prepare the blood smear: A drop of blood is spread thinly on a glass slide and allowed to air dry.
- Apply Field Stain A: The dried smear is immersed in Field Stain A for a short duration, typically around 5-10 seconds.
- Rinse: The slide is briefly rinsed in water to remove excess stain.
- Apply Field Stain B: The smear is then immersed in Field Stain B for another short duration, around 5-10 seconds.
- Rinse and dry: The slide is rinsed again in water and allowed to air dry.
- Outcome: The resulting stained smear allows for the differentiation of various cell components, making it easier to identify and analyze blood cells and any parasitic infections like malaria.
Description
Uses of Field Stain A and B
1. Malaria Diagnosis
- Identification of Malaria Parasites: Field Stains A and B are extensively used for the rapid detection of malaria parasites in blood smears. The stains highlight the parasites’ presence within red blood cells, aiding in quick diagnosis.
2. Hematological Studies
- Differentiation of Blood Cells: These stains help in differentiating various types of blood cells. For instance, they distinguish between white blood cells, red blood cells, and platelets, which is crucial for diagnosing blood disorders and conducting routine blood examinations.
3. Rapid Staining in Field Conditions
- Field and Remote Laboratory Use: Due to their simplicity and quick staining process, Field Stains A and B are particularly useful in remote and field conditions where advanced laboratory equipment might not be available.
4. Microbial Identification
- Detection of Microorganisms: While not as commonly used as other stains like Gram stain, Field Stains can sometimes aid in identifying microorganisms in blood samples or other clinical specimens.
5. Veterinary Medicine
- Animal Blood Analysis: Similar to human medicine, these stains are used in veterinary laboratories to examine blood smears from animals, aiding in the diagnosis of blood-borne diseases and overall health assessment of animals.
6. Education and Training
- Teaching Tool: Field Stains A and B are used in educational settings to teach students and trainees about blood smear preparation and the identification of different cell types and parasites.
7. Clinical Research
- Research Studies: Researchers use these stains to study the morphology of blood cells and parasites, contributing to the development of new diagnostic techniques and treatments for blood-borne diseases.
Procedure for Laboratory Use
- Sample Collection: Obtain a blood sample via finger prick or venipuncture.
- Blood Smear Preparation: Place a drop of blood on a clean glass slide and spread it thinly using another slide to create a smear.
- Drying: Allow the smear to air dry completely.
- Staining:
- Immerse the smear in Field Stain A for about 5-10 seconds.
- Briefly rinse the slide in clean water.
- Immerse the smear in Field Stain B for another 5-10 seconds.
- Rinse the slide again in clean water.
- Drying and Examination: Let the slide air dry and then examine it under a microscope.
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