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Aluminum Sulphate 500gm
Original price was: KSh750.00.KSh600.00Current price is: KSh600.00.
Ammonium Dichromate 500gm
Original price was: KSh2,500.00.KSh2,300.00Current price is: KSh2,300.00.
Ammonia Acetate
Original price was: KSh700.00.KSh600.00Current price is: KSh600.00.
Ammonium acetate (NH4C2H3O2) is a chemical compound with various applications and properties. Here are some key points about it:
Properties
- Chemical Formula: NH4C2H3O2
- Molecular Weight: 77.08 g/mol
- Appearance: White, crystalline solid
- Solubility: Highly soluble in water
- Melting Point: Decomposes upon heating
Description
Uses of Ammonia Acetate
Buffer Solutions
- pH Control: Ammonium acetate is used to prepare buffer solutions, which help maintain a stable pH in biochemical and molecular biology experiments. It is especially useful in high-performance liquid chromatography (HPLC) and capillary electrophoresis.
- LC-MS: In liquid chromatography-mass spectrometry (LC-MS), ammonium acetate buffers are commonly used because they are volatile and do not leave residue, which is crucial for sensitive detection.
Protein and Nucleic Acid Purification
- Protein Precipitation: Ammonium acetate is used to precipitate proteins from solutions, aiding in the purification and analysis of proteins.
- DNA and RNA Precipitation: It is employed in the precipitation of nucleic acids during DNA and RNA extraction procedures, often in combination with ethanol or isopropanol.
Molecular Biology
- PCR and Enzyme Reactions: Ammonium acetate is used in polymerase chain reaction (PCR) and other enzymatic reactions where a stable pH is required.
Analytical Chemistry
- Solvent for Analytical Reactions: It acts as a solvent or reagent in various analytical chemistry techniques, particularly those involving complexation reactions.
Histology
- Fixative: It can be used as a fixative in histology for tissue preparation before microscopic examination.
Electrophoresis
- Running Buffer: Ammonium acetate can be used as a running buffer in electrophoresis, aiding in the separation of biomolecules like proteins and nucleic acids based on their size and charge.
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