-8%

Sodium acetate anhydrous 99% 500gms

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Sodium acetate, with the chemical formula CH3COONa, is a versatile compound commonly used in laboratories for various applications.

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Sodium acetate

  1. Buffer Solutions:
    • pH Stabilization: Sodium acetate is a key component in buffer solutions, especially acetate buffers, which are used to maintain a stable pH in biochemical and chemical experiments. These buffers are particularly effective in the pH range of 3.6 to 5.6.
    • Applications: Commonly used in enzyme reactions, cell culture media, and other biological assays requiring a specific pH environment.
  2. Precipitation of Nucleic Acids:
    • DNA/RNA Isolation: Sodium acetate is used in the precipitation of DNA and RNA from aqueous solutions. By adding sodium acetate and alcohol (ethanol or isopropanol), nucleic acids can be precipitated and separated from the solution, facilitating their purification and concentration.
    • Procedure: After adding sodium acetate and alcohol to the nucleic acid solution, the mixture is often cooled, centrifuged, and the precipitated nucleic acids are collected.
  3. Chemical Synthesis:
    • Source of Acetate Ions: Sodium acetate provides acetate ions for various organic synthesis reactions.
    • Applications: Used in the synthesis of esters, acetylation reactions, and as a catalyst in certain organic transformations.
  4. Heat Packs:
    • Exothermic Crystallization: Sodium acetate is used in reusable heat packs. A supersaturated solution of sodium acetate releases heat upon crystallization, which can be triggered by flexing a metal disc within the pack.
    • Mechanism: The solution remains liquid until the disc is clicked, initiating crystallization and an exothermic reaction that releases heat.
  5. pH Adjustment:
    • Solution Preparation: Sodium acetate is used to adjust the pH of various solutions in chemical and biological protocols.
    • Control: It is often added to acidic or basic solutions to achieve a desired pH level.
  6. Electrophoresis:
    • Buffer Preparation: In electrophoresis, sodium acetate can be used to prepare running buffers, particularly for RNA electrophoresis.
    • Function: Helps maintain the ionic strength and pH of the buffer system during the separation process.
  7. Titration:
    • Analytical Reagent: Sodium acetate is employed as a reagent in titration methods, such as complexometric titrations involving EDTA.
    • Role: Acts as a buffering agent to maintain a stable pH during the titration process.
  8. Food Preservation (Industrial Use):
    • Preservative: Although not strictly a laboratory use, sodium acetate is also used as a preservative in the food industry to inhibit microbial growth and as a flavoring agent (E262).
    • Applications: Helps in extending the shelf life of various food products.
SKU: ACS73742CHEM0 Category:

safety precautions

  1. Personal Protective Equipment (PPE):
    • Safety Goggles: Protect your eyes from splashes.
    • Lab Coat: Wear a lab coat to protect your skin and clothing.
    • Gloves: Use chemical-resistant gloves to prevent skin contact.
    • Face Mask: If dust or aerosols are generated, consider wearing a face mask or respirator.
  2. Handling and Storage:
    • Ventilation: Work in a well-ventilated area or under a chemical fume hood to avoid inhalation of dust or vapors.
    • Storage: Store sodium acetate in a tightly sealed container, away from incompatible substances such as strong acids and oxidizing agents. Keep it in a cool, dry place.
  3. Spill Response:
    • Containment: In case of a spill, contain and collect the material using a dry, clean shovel or broom. Avoid creating dust.
    • Cleanup: Clean the spill area thoroughly with water after collecting the bulk material.
  4. First Aid Measures:
    • Inhalation: If inhaled, move to fresh air. Seek medical attention if symptoms persist.
    • Skin Contact: In case of skin contact, wash the affected area with plenty of soap and water. Remove contaminated clothing and seek medical advice if irritation occurs.
    • Eye Contact: If sodium acetate gets into the eyes, rinse immediately with plenty of water for at least 15 minutes and seek medical attention.
    • Ingestion: If ingested, rinse mouth with water. Do not induce vomiting and seek medical attention immediately.
  5. Fire Safety:
    • Fire Extinguishing: Sodium acetate is not highly flammable, but in case of a fire, use water spray, dry chemical, carbon dioxide, or appropriate foam to extinguish.
    • Firefighting Gear: Firefighters should wear appropriate protective equipment, including self-contained breathing apparatus.
  6. Waste Disposal:
    • Disposal: Dispose of sodium acetate according to local regulations. Do not release it into the environment.
    • Labeling: Clearly label waste containers and ensure they are properly sealed before disposal.
  7. General Safety Practices:
    • Avoid Eating/Drinking: Do not eat, drink, or smoke while handling chemicals.
    • Wash Hands: Wash hands thoroughly after handling sodium acetate, even if gloves were worn.
    • Training: Ensure that all personnel handling sodium acetate are trained in its proper use and aware of the associated risks.

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  1. Nandwa

    Its highly soluble in water

    Nandwa