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Sodium Sulphate Anhydrous 500g
Original price was: KSh2,500.00.KSh2,300.00Current price is: KSh2,300.00.
Sulphuric Acid 2.5 liters
Original price was: KSh1,010.00.KSh950.00Current price is: KSh950.00.
Sudan 3 Solution 2.5 liters
Original price was: KSh750.00.KSh600.00Current price is: KSh600.00.
Chemical Name: 1-(2,5-Dimethyl-4-phenylazo)-2-naphthol
Molecular Formula: C22H16N2O
Appearance: Red to brownish-red powder
Solubility: Soluble in ethanol, benzene, and oils; slightly soluble in water
Preparation of Sudan III Solution:
- Stock Solution: Typically, a stock solution is prepared by dissolving 0.5g of Sudan III in 100ml of 70% ethanol.
- Working Solution: For staining, the stock solution can be diluted with ethanol or isopropanol to the desired concentration. A common working concentration is 0.5% Sudan III in ethanol or isopropanol.
Description
Uses of Sudan 3 Solution:
- Histology:
- Lipid Staining in Tissue Sections: Sudan III stains lipids in tissue sections, especially in frozen sections, which helps in identifying and visualizing lipid droplets within cells and tissues. This is useful for diagnosing conditions like fatty liver disease and lipid storage disorders.
- Cytology:
- Cell Smears: It stains lipids in cell smears, aiding in examining cells for lipid content. This is important in diagnosing metabolic conditions or infections involving lipid accumulation.
- Forensic Pathology:
- Detection of Fat Emboli: Sudan III is used to detect fat emboli in tissues, which can be crucial in cases of trauma, decompression sickness, or other conditions where fat emboli may be present.
- Plant Biology:
- Analysis of Seed Lipids: In plant biology, Sudan III stains lipids in seeds and other plant tissues, helping in the study of lipid storage and metabolism in plants.
- Microbiology:
- Staining of Bacterial Lipids: Sudan III can stain certain types of bacteria that contain lipid-rich cell walls, aiding in their identification and characterization.
- Clinical Diagnostics:
- Identifying Lipid-Rich Deposits: It is used in clinical settings to identify lipid-rich deposits in various tissues, indicative of diseases such as atherosclerosis or lipid storage diseases.
- Veterinary Medicine:
- Lipid Analysis in Animal Tissues: Sudan III can be used to analyze lipid content in animal tissues, assisting in diagnosing lipid-related conditions in veterinary practice.
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